Literature DB >> 3753430

The sensitivity of isoelectric focusing and electrophoresis in the detection of sequence differences in proteins.

T McLellan, L S Inouye.   

Abstract

Fourteen myoglobins of known sequence were examined by isoelectric focusing with and without urea. The 14 sequences formed six distinct mobility classes on gels without urea and three classes on those with urea. For these proteins, isoelectric focusing provides no advantage over single, nonequilibrium, nondenaturing gels in the total number of distinguishable mobility classes. Only major charge differences, resulting from the changes in the total numbers of acidic and basic amino acids, can be detected on gels with urea, indicating that denaturation by urea alters proteins so that small differences in ionization are eliminated.

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Year:  1986        PMID： 3753430     DOI： 10.1007/bf00504335

Source DB:  PubMed          Journal:  Biochem Genet        ISSN： 0006-2928            Impact factor:   1.890


  22 in total

1.  Reevaluation of level of genic heterozygosity in natural population of Drosophila melanogaster by two-dimensional electrophoresis.

Authors:  A J Brown; C H Langley
Journal:  Proc Natl Acad Sci U S A       Date:  1979-05       Impact factor: 11.205

2.  Identification of a new serum protein polymorphism as transferrin.

Authors:  M Thymann
Journal:  Hum Genet       Date:  1978-08-31       Impact factor: 4.132

Review 3.  Protein denaturation.

Authors:  C Tanford
Journal:  Adv Protein Chem       Date:  1968

4.  The analysis of hidden electrophoretic variation: interspecific electrophoretic differentiation and amino acid divergence.

Authors:  P A Fuerst; R E Ferrell
Journal:  J Mol Evol       Date:  1983       Impact factor: 2.395

5.  Two previously undetected variants of glutamic-pyruvic transaminase found by acidic polyacrylamide gel electrophoresis.

Authors:  T McLellan
Journal:  Am J Hum Genet       Date:  1982-07       Impact factor: 11.025

6.  Group-specific component: evidence for two subtypes of the Gc1 gene.

Authors:  J Constans; M Viau
Journal:  Science       Date:  1977-12-09       Impact factor: 47.728

7.  Identification of human red cell glutamate-pyruvate transaminase (GPT) phenotypes by isoelectric focusing.

Authors:  C F Pelzer; R A Norum
Journal:  Am J Hum Genet       Date:  1985-01       Impact factor: 11.025

8.  Human heterozygosity: a new estimate.

Authors:  E H McConkey; B J Taylor; D Phan
Journal:  Proc Natl Acad Sci U S A       Date:  1979-12       Impact factor: 11.205

9.  Properdin factor B (Bf) polymorphism: subtyping of SS phenotypes.

Authors:  V David; R Fauchet; H Phengsavath; L Guenet; J Y Le Gall
Journal:  Hum Genet       Date:  1983       Impact factor: 4.132

10.  Molecular charge and electrophoretic mobility in cetacean myoglobins of known sequence.

Authors:  T McLellan
Journal:  Biochem Genet       Date:  1984-02       Impact factor: 1.890
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  3 in total

1.  The influence of site-specificity of single amino acid substitutions on electrophoretic separation of yeast iso-1-cytochrome c.

Authors:  T McLellan; F Sherman
Journal:  J Mol Evol       Date:  1991-10       Impact factor: 2.395

2.  Comparisons of genetic variability detected among mouse blood proteins using one- and two-dimensional electrophoreses.

Authors:  T W Sands; K A Hill; M L Petras
Journal:  Biochem Genet       Date:  1990-10       Impact factor: 1.890

3.  Molecular evolution among some Drosophila species groups as indicated by two-dimensional electrophoresis.

Authors:  G S Spicer
Journal:  J Mol Evol       Date:  1988       Impact factor: 2.395
  3 in total

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