Literature DB >> 3748820

Caenorhabditis elegans DNA does not contain 5-methylcytosine at any time during development or aging.

V J Simpson, T E Johnson, R F Hammen.   

Abstract

DNA, isolated from age-synchronous senescent populations of Caenorhabditis elegans has been quantitatively and qualitatively analyzed for the presence of 5-methylcytosine. High performance liquid chromatography on two wild-type and several mutant strains of C. elegans failed to detect any 5-methylcytosine. The restriction endonuclease isoschizomers, HpaII and MspI, were used to digest genomic DNA after CsCl purification and failed to detect any 5' cytosine methylation at any age. We conclude that C. elegans does not contain detectable (0.01 mole percent) levels of 5-methylcytosine.

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Year:  1986        PMID： 3748820      PMCID： PMC311675          DOI： 10.1093/nar/14.16.6711

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  19 in total

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