Literature DB >> 8493087

Synthesis and antibody-mediated detection of oligonucleotides containing multiple 2,4-dinitrophenyl reporter groups.

J Grzybowski1, D W Will, R E Randall, C A Smith, T Brown.   

Abstract

A series of non-nucleoside-based 2,4-dinitrophenyl (DNP) phosphoramidites have been prepared and used in the multiple labelling of oligonucleotides during solid-phase synthesis. The length of spacer arm between the DNP label and the oligonucleotide phosphate backbone, and the number of attached DNP groups have both been varied in order to determine the optimum conditions for anti-DNP antibody binding. Detection using enzyme-linked colorimetric techniques showed sensitivity equivalent to that obtainable using biotinylated oligonucleotides.

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Year:  1993        PMID： 8493087      PMCID： PMC309404          DOI： 10.1093/nar/21.8.1705

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  22 in total

1.  Non-radioactive labeling and detection of nucleic acids. II. Optimization of the digoxigenin system.

Authors:  H J Höltke; R Seibl; J Burg; K Mühlegger; C Kessler
Journal:  Biol Chem Hoppe Seyler       Date:  1990-10

2.  Non-radioactive labeling and detection of nucleic acids. IV. Synthesis and properties of digoxigenin-modified 2'-deoxyuridine-5'-triphosphates and a photoactivatable analog of digoxigenin (photodigoxigenin).

Authors:  K Mühlegger; E Huber; H von der Eltz; R Rüger; C Kessler
Journal:  Biol Chem Hoppe Seyler       Date:  1990-10

3.  Non-radioactive labeling and detection of nucleic acids. I. A novel DNA labeling and detection system based on digoxigenin: anti-digoxigenin ELISA principle (digoxigenin system).

Authors:  C Kessler; H J Höltke; R Seibl; J Burg; K Mühlegger
Journal:  Biol Chem Hoppe Seyler       Date:  1990-10

4.  The synthesis of oligonucleotides that contain 2,4-dinitrophenyl reporter groups.

Authors:  D W Will; C E Pritchard; T Brown
Journal:  Carbohydr Res       Date:  1991-09-02       Impact factor: 2.104

5.  Non-radioactive labeling and detection of nucleic acids. III. Applications of the digoxigenin system.

Authors:  R Seibl; H J Höltke; R Rüger; A Meindl; H G Zachau; R Rasshofer; M Roggendorf; H Wolf; N Arnold; J Wienberg
Journal:  Biol Chem Hoppe Seyler       Date:  1990-10

6.  One-step labelling of oligonucleotides with fluoresceine during automated synthesis.

Authors:  F Schubert; K Ahlert; D Cech; A Rosenthal
Journal:  Nucleic Acids Res       Date:  1990-06-11       Impact factor: 16.971

7.  Synthesis of 8-(2-4 dinitrophenyl 2-6 aminohexyl) amino-adenosine 5' triphosphate: biological properties and potential uses.

Authors:  C Vincent; P Tchen; M Cohen-Solal; P Kourilsky
Journal:  Nucleic Acids Res       Date:  1982-11-11       Impact factor: 16.971

8.  Site specific functionalization of oligonucleotides for attaching two different reporter groups.

Authors:  S Agrawal; P C Zamecnik
Journal:  Nucleic Acids Res       Date:  1990-09-25       Impact factor: 16.971

9.  Biotinyl and phosphotyrosinyl phosphoramidite derivatives useful in the incorporation of multiple reporter groups on synthetic oligonucleotides.

Authors:  K Misiura; I Durrant; M R Evans; M J Gait
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

10.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.

Authors:  P R Langer; A A Waldrop; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205
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  2 in total

1.  A comparative study of digoxigenin, 2,4-dinitrophenyl, and alkaline phosphatase as deoxyoligonucleotide labels in non-radioisotopic in situ hybridisation.

Authors:  S J Harper; E Bailey; C M McKeen; A S Stewart; J H Pringle; J Feehally; T Brown
Journal:  J Clin Pathol       Date:  1997-08       Impact factor: 3.411

2.  Branched poly-labelled oligonucleotides: enhanced specificity of fork-shaped biotinylated oligoribonucleotides for antisense affinity selection.

Authors:  S Teigelkamp; S Ebel; D W Will; T Brown; J D Beggs
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971
  2 in total

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