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Literature DB >> 373354

In vitro conversion of proinsulin to insulin by cathepsin B and role of C-peptide.

R B Puri, K Anjaneyulu, J R Kidwai, V K Mohan Rao.

Abstract

Cathepsin B, purified from isolated islets of Langerhans, when incubated with proinsulin under in vitro conditions could convert proinsulin to insulin and C-peptide, releasing free arginine and lysine. When C-peptide, prepared from rat pancreas, was added to the incubation system consisting of proinsulin and cathepsin B, it completely inhibited the conversion of proinsulin to insulin.

Entities: Chemical Gene Species

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Year: 1978 PMID： 373354 DOI： 10.1007/bf02590747

Source DB: PubMed Journal: Acta Diabetol Lat ISSN： 0001-5563

22 in total

1. A spectrophotometric determination of trypsin and chymotrypsin.

Authors: G W SCHWERT; Y TAKENAKA
Journal: Biochim Biophys Acta Date: 1955-04

2. The activation of trypsinogen by cathepsin B.

Authors: L M GREENBAUM; A HIRSHKOWITZ; I SHOICHET
Journal: J Biol Chem Date: 1959-11 Impact factor: 5.157

3. The effect of fluoride on the succinic oxidase system.

Authors: E C SLATER; W D BORNER
Journal: Biochem J Date: 1952-10 Impact factor: 3.857

4. Evidence for the presence of insulin in blood serum; a method for an approximate determination of the insulin content of blood.

Authors: J GROEN; C E KAMMINGA; A F WILLEBRANDS; J R BLICKMAN
Journal: J Clin Invest Date: 1952-01 Impact factor: 14.808

3. In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies.

Authors: R Bansal; N Ahmad; J R Kidwai
Journal: Acta Diabetol Lat Date: 1980 Jul-Dec

4. Insulinotropic action of L-rhamnose in isolated rat islets.

Authors: A K Chatterjee; S K Mukherjee
Journal: Acta Diabetol Lat Date: 1981 Jul-Sep

5. Stimulation by prostaglandin E2 of a high-affinity GTPase in the secretory granules of normal rat and human pancreatic islets.

Authors: A Kowluru; S A Metz
Journal: Biochem J Date: 1994-01-15 Impact factor: 3.857

6. Characterization of proinsulin- and proglucagon-converting activities in isolated islet secretory granules.

Authors: D J Fletcher; J P Quigley; G E Bauer; B D Noe
Journal: J Cell Biol Date: 1981-08 Impact factor: 10.539

6 in total

In vitro conversion of proinsulin to insulin by cathepsin B and role of C-peptide.

1. A spectrophotometric determination of trypsin and chymotrypsin.

2. The activation of trypsinogen by cathepsin B.

3. The effect of fluoride on the succinic oxidase system.

4. Evidence for the presence of insulin in blood serum; a method for an approximate determination of the insulin content of blood.

5. Isolation of a proinsulin connecting peptide fragment (C-peptide) from bovine and human pancreas.

6. On the biosynthesis, intracellular transport and mechanism of conversion of proinsulin to insulin and C-peptide.

7. Porcine proinsulin: characterization and amino acid sequence.

8. Cathepsin B, the lysosomal thiol proteinase of calf liver.

9. Proinsulin secretion by a pancreatic beta-cell adenoma. Proinsulin and C-peptide secretion.

10. THE ESTIMATION OF PEPSIN, TRYPSIN, PAPAIN, AND CATHEPSIN WITH HEMOGLOBIN.

Review 1. Multiple forms of gastroenteropancreatic hormones.

2. Identification of a 31,500 molecular weight islet cell protease as cathepsin B.

3. In vitro conversion of proinsulin to insulin by cathepsin B in isolated islets and its inhibition by cathepsin B antibodies.

4. Insulinotropic action of L-rhamnose in isolated rat islets.

5. Stimulation by prostaglandin E2 of a high-affinity GTPase in the secretory granules of normal rat and human pancreatic islets.

6. Characterization of proinsulin- and proglucagon-converting activities in isolated islet secretory granules.