| Literature DB >> 3717554 |
L A Idahl, P E Sandström, J Sehlin.
Abstract
A single-step assay for serum glucose measurements is described. The assay is based on the phosphorylation of D-glucose by glucokinase and the measurement of ATP consumption by firefly luciferase. The luminescence is recorded in an ordinary liquid scintillation spectrometer. The use of stable reagents and a stable final signal (light emission) makes it possible to analyze a large number of samples in each assay run. The assay is of particular value when repeated serum glucose determinations are performed on samples from small laboratory animals.Entities:
Mesh：
Substances：
Year: 1986 PMID： 3717554 DOI： 10.1016/0003-2697(86)90243-5
Source DB: PubMed Journal: Anal Biochem ISSN： 0003-2697 Impact factor: 3.365