Literature DB >> 3714487

Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance.

J A Vara, A Portela, J Ortín, A Jiménez.   

Abstract

The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector. When this construction was introduced into VERO cells it expressed the relevant enzymic activity. Moreover, the puromycin N-acetyl transferase gene has been used as a dominant marker for the selection of transformed mammalian cells able to grow in the presence of the antibiotic.

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Year:  1986        PMID： 3714487      PMCID： PMC311469          DOI： 10.1093/nar/14.11.4617

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors:  F L Graham; A J van der Eb
Journal:  Virology       Date:  1973-04       Impact factor: 3.616

2.  Extraction of nucleic acids from agarose gels.

Authors:  J Langridge; P Langridge; P L Bergquist
Journal:  Anal Biochem       Date:  1980-04       Impact factor: 3.365

Review 3.  Gene cloning in Streptomyces.

Authors:  K F Chater; D A Hopwood; T Kieser; C J Thompson
Journal:  Curr Top Microbiol Immunol       Date:  1982       Impact factor: 4.291

4.  SV40-transformed simian cells support the replication of early SV40 mutants.

Authors:  Y Gluzman
Journal:  Cell       Date:  1981-01       Impact factor: 41.582

5.  Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

Authors:  P J Southern; P Berg
Journal:  J Mol Appl Genet       Date:  1982

6.  Isolation and characterization of puromycin-resistant clones from cultured mosquito cells.

Authors:  A M Fallon; V Stollar
Journal:  Somatic Cell Genet       Date:  1982-07

7.  DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

Authors:  M Wigler; A Pellicer; S Silverstein; R Axel; G Urlaub; L Chasin
Journal:  Proc Natl Acad Sci U S A       Date:  1979-03       Impact factor: 11.205

8.  Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.

Authors:  C M Gorman; L F Moffat; B H Howard
Journal:  Mol Cell Biol       Date:  1982-09       Impact factor: 4.272

9.  Biosynthesis of puromycin by Streptomyces alboniger: characterization of puromycin N-acetyltransferase.

Authors:  J Vara; J A Perez-Gonzalez; A Jimenez
Journal:  Biochemistry       Date:  1985-12-31       Impact factor: 3.162

10.  Genetic variability of Hong Kong (H3N2) influenza viruses: spontaneous mutations and their location in the viral genome.

Authors:  J Ortín; R Nájera; C López; M Dávila; E Domingo
Journal:  Gene       Date:  1980-11       Impact factor: 3.688
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  59 in total

1.  Selection of RNA replicons capable of persistent noncytopathic replication in mammalian cells.

Authors:  I Frolov; E Agapov; T A Hoffman; B M Prágai; M Lippa; S Schlesinger; C M Rice
Journal:  J Virol       Date:  1999-05       Impact factor: 5.103

2.  Use of puromycin N-acetyltransferase (PAC) as a new reporter gene in transgenic animals.

Authors:  E Gómez Lahoz; M S López de Haro; A Nieto; P Esponda
Journal:  Nucleic Acids Res       Date:  1991-06-25       Impact factor: 16.971

3.  Development of an in vivo assay to identify structural determinants in murine leukemia virus reverse transcriptase important for fidelity.

Authors:  E K Halvas; E S Svarovskaia; V K Pathak
Journal:  J Virol       Date:  2000-01       Impact factor: 5.103

4.  Expression and maturation of human cathepsin D in baby-hamster kidney cells.

Authors:  M Horst; A Hasilik
Journal:  Biochem J       Date:  1991-01-15       Impact factor: 3.857

5.  Relative rates of retroviral reverse transcriptase template switching during RNA- and DNA-dependent DNA synthesis.

Authors:  R R Bowman; W S Hu; V K Pathak
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

6.  Selection for retroviral insertions into regulated genes.

Authors:  J A Gogos; W Lowry; M Karayiorgou
Journal:  J Virol       Date:  1997-02       Impact factor: 5.103

7.  Gene transfer into mammalian cells by a Rous sarcoma virus-based retroviral vector with the host range of the amphotropic murine leukemia virus.

Authors:  E V Barsov; S H Hughes
Journal:  J Virol       Date:  1996-06       Impact factor: 5.103

8.  Effect of a null mutation of the insulin-like growth factor I receptor gene on growth and transformation of mouse embryo fibroblasts.

Authors:  C Sell; G Dumenil; C Deveaud; M Miura; D Coppola; T DeAngelis; R Rubin; A Efstratiadis; R Baserga
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

9.  Reversion of autocrine transformation by a dominant negative platelet-derived growth factor mutant.

Authors:  F S Vassbotn; M Andersson; B Westermark; C H Heldin; A Ostman
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272

10.  Parvoviruses are inefficient in inducing interferon-beta, tumor necrosis factor-alpha, or interleukin-6 in mammalian cells.

Authors:  J R Schlehofer; M Rentrop; D N Männel
Journal:  Med Microbiol Immunol       Date:  1992       Impact factor: 3.402
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