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Literature DB >> 3663125

Unusual ciliate-specific codons in Tetrahymena mRNAs are translated correctly in a rabbit reticulocyte lysate supplemented with a subcellular fraction from Tetrahymena.

P H Andreasen¹, H Dreisig, K Kristiansen.

Abstract

The codon usage of Tetrahymena thermophila and other ciliates deviates from the 'universal genetic code' in that UAA and probably UAG are not translational termination signals but code for glutamine. Therefore, translation in vitro of mRNA from Tetrahymena in a reticulocyte lysate is prematurely terminated if a UAA or UAG triplet is present in the reading frame of the mRNA. We show that the addition of a subcellular fraction from Tetrahymena thermophila enables a rabbit reticulocyte lysate to translate Tetrahymena mRNAs into full-sized proteins. The activity of the subcellular fraction is shown to depend on the combined function of a protein component(s) and a tRNA(s). The subcellular fraction is easily prepared and its usefulness for the identification of isolated mRNAs from Tetrahymena by their translation products in vitro is demonstrated.

Entities: Chemical Species

Mesh：

Substances：

Year: 1987 PMID： 3663125 PMCID： PMC1147995 DOI： 10.1042/bj2440331

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

19 in total

1. Nucleotide sequence of a macronuclear DNA molecule coding for alpha-tubulin from the ciliate Stylonychia lemnae. Special codon usage: TAA is not a translation termination codon.

Authors: E Helftenbein
Journal: Nucleic Acids Res Date: 1985-01-25 Impact factor: 16.971

2. Yeast suppressors of UAA and UAG nonsense codons work efficiently in vitro via tRNA.

Authors: R F Gesteland; M Wolfner; P Grisafi; G Fink; D Botstein; J R Roth
Journal: Cell Date: 1976-03 Impact factor: 41.582

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

4. An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors: H R Pelham; R J Jackson
Journal: Eur J Biochem Date: 1976-08-01

5. Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis.

Authors: E T David; K E Smith
Journal: Biochem J Date: 1981-03-15 Impact factor: 3.857

6. Regulation of ribosome synthesis in Tetrahymena pyriformis. 1. Coordination of synthesis of ribosomal proteins and ribosomal RNA during nutritional shift-down.

Authors: H Dreisig; P H Andreasen; K Kristiansen
Journal: Eur J Biochem Date: 1984-05-02

7. Regulation of ribosome synthesis in Tetrahymena pyriformis. 3. Analysis by translation in vitro of RNA isolated during nutritional shift-down and nutritional shift-up.

Authors: P H Andreasen; H Dreisig; K Kristiansen
Journal: Eur J Biochem Date: 1984-05-02

2. Cloning and characterization of the major histone H2A genes completes the cloning and sequencing of known histone genes of Tetrahymena thermophila.

Authors: X Liu; M A Gorovsky
Journal: Nucleic Acids Res Date: 1996-08-01 Impact factor: 16.971

3. Differential use of termination codons in ciliated protozoa.

Authors: D S Harper; C L Jahn
Journal: Proc Natl Acad Sci U S A Date: 1989-05 Impact factor: 11.205

4. Either of the major H2A genes but not an evolutionarily conserved H2A.F/Z variant of Tetrahymena thermophila can function as the sole H2A gene in the yeast Saccharomyces cerevisiae.

Authors: X Liu; J Bowen; M A Gorovsky
Journal: Mol Cell Biol Date: 1996-06 Impact factor: 4.272

4 in total