Literature DB >> 6796065

Preparation and partial characterization of cell-free protein-synthesizing extracts from Tetrahymena pyriformis.

E T David, K E Smith.   

Abstract

1. We have examined methods necessary for preparing post-mitochondrial supernatants from Tetrahymena pyriformis strain HSM that are capable of efficient cell-free protein synthesis. 2. The requirements for optimum synthesis in these extracts are described. 3. Data relating to the kinetics of protein synthesis and the initiation capacity of these supernatants are presented.

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Year:  1981        PMID： 6796065      PMCID： PMC1162811          DOI： 10.1042/bj1940761

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  9 in total

1.  Inhibition at the initiation level of eukaryotic protein synthesis by T-2 toxin.

Authors:  K E Smith; M Cannon; E Cundliffe
Journal:  FEBS Lett       Date:  1975-01-15       Impact factor: 4.124

2.  The analysis of intermediary reactions involved in protein synthesis, in a cell-free extract of Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.

Authors:  E Gasior; F Herrera; C S McLaughlin; K Moldave
Journal:  J Biol Chem       Date:  1979-05-25       Impact factor: 5.157

3.  Growth-dependent factors in the regulation of aminoacyl-tRNA synthetase activities of Tetrahymena pyriformis.

Authors:  B Chua; C Elson; E Shrago
Journal:  Biochim Biophys Acta       Date:  1977-10-18

4.  Protein synthesis in a postmitochondrial supernatant system from rat liver. An effect of diabetes at the level of peptide chain initiation.

Authors:  V M Pain
Journal:  FEBS Lett       Date:  1973-09-01       Impact factor: 4.124

5.  An efficient mRNA-dependent translation system from reticulocyte lysates.

Authors:  H R Pelham; R J Jackson
Journal:  Eur J Biochem       Date:  1976-08-01

6.  Protein synthesis in vitro with Tetrahymena mitochondrial ribosomes.

Authors:  N E Allen; Y Suyama
Journal:  Biochim Biophys Acta       Date:  1972-02-15

7.  Protein synthesis in a cell-free preparation from Tetrahymena pyriformis GL.

Authors:  K A Conklin; S C Chou
Journal:  Comp Biochem Physiol B       Date:  1971-12-15

8.  Structural requirements for the inhibitory action of 12,13-epoxytrichothecenes on protein synthesis in eukaryotes.

Authors:  C J Carter; M Cannon
Journal:  Biochem J       Date:  1977-09-15       Impact factor: 3.857

9.  The preparation and characterization of a cell-free system from Saccharomyces cerevisiae that translates natural messenger ribonucleic acid.

Authors:  E Gasior; F Herrera; I Sadnik; C S McLaughlin; K Moldave
Journal:  J Biol Chem       Date:  1979-05-25       Impact factor: 5.157
  9 in total
  4 in total

1.  mRNAs for the immobilization antigens of Paramecium.

Authors:  J R Preer; L B Preer; B M Rudman
Journal:  Proc Natl Acad Sci U S A       Date:  1981-11       Impact factor: 11.205

2.  Initiation of protein synthesis in cell-free extracts of Tetrahymena pyriformis.

Authors:  K E Smith; E T David
Journal:  Biochem J       Date:  1981-05-15       Impact factor: 3.857

3.  Unusual ciliate-specific codons in Tetrahymena mRNAs are translated correctly in a rabbit reticulocyte lysate supplemented with a subcellular fraction from Tetrahymena.

Authors:  P H Andreasen; H Dreisig; K Kristiansen
Journal:  Biochem J       Date:  1987-06-01       Impact factor: 3.857

4.  Exceptional codon recognition by the glutamine tRNAs in Saccharomyces cerevisiae.

Authors:  I Edelman; M R Culbertson
Journal:  EMBO J       Date:  1991-06       Impact factor: 11.598
  4 in total

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