Literature DB >> 3644239

Assembly of pre-mRNA splicing complex is cap dependent.

E Patzelt, E Thalmann, K Hartmuth, D Blaas, E Kuechler.   

Abstract

To study the influence of the ubiquitous cap structure of nuclear pre-mRNAs on the assembly of a functional splicing complex, the in vitro splicing of a truncated human metallothionein pre-mRNA was examined in the presence of the cap analogue m7GTP. Significant inhibition of splicing was observed at a concentration as low as 5 microM m7GTP. Analysis of the splicing reaction on glycerol density gradients showed two complexes sedimenting at 45S and 22S. When the reaction was carried out in presence of m7GTP a marked decrease of the material sedimenting at 45S, representing the active splicing complex, was observed. When capped pre-mRNA was replaced by uncapped pre-mRNA, complex formation was significantly reduced. These data indicate that the cap structure plays an important yet unknown role in the assembly of spliceosomes.

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Year:  1987        PMID： 3644239      PMCID： PMC340556          DOI： 10.1093/nar/15.4.1387

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  20 in total

1.  Electrophoretic separation of complexes involved in the splicing of precursors to mRNAs.

Authors:  M M Konarska; P A Sharp
Journal:  Cell       Date:  1986-09-12       Impact factor: 41.582

2.  Excision of an intact intron as a novel lariat structure during pre-mRNA splicing in vitro.

Authors:  B Ruskin; A R Krainer; T Maniatis; M R Green
Journal:  Cell       Date:  1984-08       Impact factor: 41.582

3.  Splicing of in vitro synthesized messenger RNA precursors in HeLa cell extracts.

Authors:  N Hernandez; W Keller
Journal:  Cell       Date:  1983-11       Impact factor: 41.582

4.  Messenger RNA splicing in vitro: an excised intervening sequence and a potential intermediate.

Authors:  P J Grabowski; R A Padgett; P A Sharp
Journal:  Cell       Date:  1984-06       Impact factor: 41.582

5.  CAP binding proteins associated with the nucleus.

Authors:  E Patzelt; D Blaas; E Kuechler
Journal:  Nucleic Acids Res       Date:  1983-09-10       Impact factor: 16.971

6.  The addition of 5' cap structures occurs early in hnRNA synthesis and prematurely terminated molecules are capped.

Authors:  M Salditt-Georgieff; M Harpold; S Chen-Kiang; J E Darnell
Journal:  Cell       Date:  1980-01       Impact factor: 41.582

7.  Splicing of adenovirus RNA in a cell-free transcription system.

Authors:  R A Padgett; S F Hardy; P A Sharp
Journal:  Proc Natl Acad Sci U S A       Date:  1983-09       Impact factor: 11.205

8.  Normal and mutant human beta-globin pre-mRNAs are faithfully and efficiently spliced in vitro.

Authors:  A R Krainer; T Maniatis; B Ruskin; M R Green
Journal:  Cell       Date:  1984-04       Impact factor: 41.582

9.  Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors:  J D Dignam; R M Lebovitz; R G Roeder
Journal:  Nucleic Acids Res       Date:  1983-03-11       Impact factor: 16.971

10.  Human metallothionein genes--primary structure of the metallothionein-II gene and a related processed gene.

Authors:  M Karin; R I Richards
Journal:  Nature       Date:  1982-10-28       Impact factor: 49.962
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  19 in total

1.  Adenovirus early region 4 stimulates mRNA accumulation via 5' introns.

Authors:  K Nordqvist; G Akusjärvi
Journal:  Proc Natl Acad Sci U S A       Date:  1990-12       Impact factor: 11.205

2.  mRNAs that mature through trans-splicing in Caenorhabditis elegans have a trimethylguanosine cap at their 5' termini.

Authors:  K Van Doren; D Hirsh
Journal:  Mol Cell Biol       Date:  1990-04       Impact factor: 4.272

3.  Processing of chimeric introns in dicot plants: evidence for a close cooperation between 5' and 3' splice sites.

Authors:  E Waigmann; A Barta
Journal:  Nucleic Acids Res       Date:  1992-01-11       Impact factor: 16.971

4.  The pre-mRNA 5' cap determines whether U6 small nuclear RNA succeeds U1 small nuclear ribonucleoprotein particle at 5' splice sites.

Authors:  L O'Mullane; I C Eperon
Journal:  Mol Cell Biol       Date:  1998-12       Impact factor: 4.272

5.  A nuclear cap binding protein from HeLa cells.

Authors:  M Ohno; N Kataoka; Y Shimura
Journal:  Nucleic Acids Res       Date:  1990-12-11       Impact factor: 16.971

6.  In vitro selection of a 7-methyl-guanosine binding RNA that inhibits translation of capped mRNA molecules.

Authors:  A A Haller; P Sarnow
Journal:  Proc Natl Acad Sci U S A       Date:  1997-08-05       Impact factor: 11.205

7.  Affinity chromatography of HeLa splicing extracts on m7 GTP-sepharose and photoaffinity-labeling of cap-binding proteins with gamma-[(4-benzoylphenyl) methylamido]-7-methyl-guanosine-5'-triphosphate.

Authors:  E Thalmann; D Blaas; E Kuechler
Journal:  Mol Biol Rep       Date:  1990       Impact factor: 2.316

8.  Identification of the factors that interact with NCBP, an 80 kDa nuclear cap binding protein.

Authors:  N Kataoka; M Ohno; I Moda; Y Shimura
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

9.  A yeast cap binding protein complex (yCBC) acts at an early step in pre-mRNA splicing.

Authors:  J D Lewis; D Görlich; I W Mattaj
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

10.  Involvement of the nuclear cap-binding protein complex in alternative splicing in Arabidopsis thaliana.

Authors:  Katarzyna Dorota Raczynska; Craig G Simpson; Adam Ciesiolka; Lukasz Szewc; Dominika Lewandowska; Jim McNicol; Zofia Szweykowska-Kulinska; John W S Brown; Artur Jarmolowski
Journal:  Nucleic Acids Res       Date:  2009-10-28       Impact factor: 16.971
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