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Literature DB >> 6828386

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Abstract

We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).

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Year: 1983 PMID： 6828386 PMCID： PMC325809 DOI： 10.1093/nar/11.5.1475

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

20 in total

1. Prolonged transcription in a cell-free system involving nuclei and cytoplasm.

Authors: G J Wu; G Zubay
Journal: Proc Natl Acad Sci U S A Date: 1974-05 Impact factor: 11.205

2. Mammalian deoxyribonucleic acid-dependent ribonucleic acid polymerases. I. Purification and properties of an -amanitin-sensitive ribonucleic acid polymerase and stimulatory factors from HeLa and KB cells.

Authors: B Sugden; W Keller
Journal: J Biol Chem Date: 1973-06-10 Impact factor: 5.157

3. Promoter sequences of eukaryotic protein-coding genes.

Authors: J Corden; B Wasylyk; A Buchwalder; P Sassone-Corsi; C Kedinger; P Chambon
Journal: Science Date: 1980-09-19 Impact factor: 47.728

4. Identification of regulatory sequences in the prelude sequences of an H2A histone gene by the study of specific deletion mutants in vivo.

Authors: R Grosschedl; M L Birnstiel
Journal: Proc Natl Acad Sci U S A Date: 1980-03 Impact factor: 11.205

5. Multiple factors are required for the accurate transcription of purified genes by RNA polymerase III.

Authors: J Segall; T Matsui; R G Roeder
Journal: J Biol Chem Date: 1980-12-25 Impact factor: 5.157

6. Accurate transcription initiation on a purified mouse beta-globin DNA fragment in a cell-free system.

Authors: D S Luse; R G Roeder
Journal: Cell Date: 1980-07 Impact factor: 41.582

7. Specific in vitro initiation of transcription on conalbumin and ovalbumin genes and comparison with adenovirus-2 early and late genes.

Authors: B Wasylyk; C Kédinger; J Corden; O Brison; P Chambon
Journal: Nature Date: 1980-06-05 Impact factor: 49.962

8. Selective and accurate initiation of transcription at the Ad2 major late promotor in a soluble system dependent on purified RNA polymerase II and DNA.

Authors: P A Weil; D S Luse; J Segall; R G Roeder
Journal: Cell Date: 1979-10 Impact factor: 41.582

9. Faithful transcription of eukaryotic genes by RNA polymerase III in systems reconstituted with purified DNA templates.

Authors: P A Weil; J Segall; B Harris; S Y Ng; R G Roeder
Journal: J Biol Chem Date: 1979-07-10 Impact factor: 5.157

10. Purification using polyethylenimine precipitation and low molecular weight subunit analyses of calf thymus and wheat germ DNA-dependent RNA polymerase II.

Authors: H G Hodo; S P Blatti
Journal: Biochemistry Date: 1977-05-31 Impact factor: 3.162

2000 in total

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Journal: Proc Natl Acad Sci U S A Date: 1999-08-17 Impact factor: 11.205

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Authors: L Jørgensen; K B Hendil
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3. Sm and Sm-like proteins assemble in two related complexes of deep evolutionary origin.

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4. Purification and initial characterization of primate satellite chromatin.

Authors: A Jasinskas; B A Hamkalo
Journal: Chromosome Res Date: 1999 Impact factor: 5.239

5. Transcription from the P2 promoter of the growth hormone receptor gene involves members of the Sp transcription factor family.

Authors: T E Adams
Journal: Biochem J Date: 1999-12-15 Impact factor: 3.857

10. Identification of a novel element involved in regulation of the lytic switch BZLF1 gene promoter of Epstein-Barr virus.

Authors: R J Kraus; S J Mirocha; H M Stephany; J R Puchalski; J E Mertz
Journal: J Virol Date: 2001-01 Impact factor: 5.103