Krzysztof Marycz1,2,3, Paulina Sobierajska4, Rafał J Wiglusz4, Rafał Idczak5, Jean-Marie Nedelec6, Andrzej Fal2, Katarzyna Kornicka-Garbowska1,3. 1. The Department of Experimental Biology, University of Environmental and Life Sciences Wroclaw, Wroclaw 50-375, Poland. 2. Collegium Medicum, Cardinal Stefan Wyszynski University in Warsaw, Warsaw 01-938, Poland. 3. International Institute of Translational Medicine, Malin 55-114, Poland. 4. Institute of Low Temperature and Structure Research, Polish Academy of Sciences, Wroclaw 50-422, Poland. 5. Centre for Advanced Materials and Smart Structures, Polish Academy of Sciences, Wroclaw 50-950, Poland. 6. CNRS, SIGMA Clermont, ICCF, Université Clermont Auvergne, Clermont-Ferrand, France.
Abstract
Purpose: The presented study aimed to investigate the effects of Fe3O4 nanoparticles and static magnetic field on osteoblast and osteoclasts' metabolic activity. Methods: Magnetic nanoparticles were prepared by a wet chemical co-precipitation process and analyzed using X-ray powder diffraction, high-resolution transmission electron microscope (HRTEM), dynamic light scattering (DLS), laser Doppler velocimetry, Raman and the Mössbauer spectroscopy. In vitro experiments were performed using MC3T3, 4B12 and RAW 264.7 cell lines. Cells were cultured in the presence of nanoparticles and with or without exposure to the magnetic field. Proteins were investigated with Western blotting and immunofluorescence and Western blot. Gene expression was analyzed with a quantitative real-time polymerase chain reaction. Results: Obtained particles were in the nano-range (average size around 50 nm) and had a spherical-like morphology. The typical hydrodynamic size was in the range 178-202 nm and Zeta potential equaled -9.51 mV. Mössbauer spectrum corresponds to the Fe+3 ions in tetrahedral (A) and Fe+3 and Fe+2 ions in octahedral (B) sites of Fe3O4. In vitro study revealed cytocompatibility and anti-inflammatory effects of fabricated nanoparticles. Furthermore, it was shown that nanoparticles combined with magnetic field exposure enhance osteogenic differentiation of MC3T3 cells by upregulation of RUNX-2 activity. Under the same experimental condition, nanoparticles and magnetic field decreased osteoclastogenesis of 4B12 by the induction of apoptosis through the mitochondrial-dependent pathway. Conclusion: Fe3O4 nanoparticles together with magnetic field can be applied for the fabrication of novel biomaterials for the treatment of bone disorders related to bone loss in which a balance between bone-forming and resorbing cells is disturbed.
Purpose: The presented study aimed to investigate the effects of Fe3O4 nanoparticles and static magnetic field on osteoblast and osteoclasts' metabolic activity. Methods: Magnetic nanoparticles were prepared by a wet chemical co-precipitation process and analyzed using X-ray powder diffraction, high-resolution transmission electron microscope (HRTEM), dynamic light scattering (DLS), laser Doppler velocimetry, Raman and the Mössbauer spectroscopy. In vitro experiments were performed using MC3T3, 4B12 and RAW 264.7 cell lines. Cells were cultured in the presence of nanoparticles and with or without exposure to the magnetic field. Proteins were investigated with Western blotting and immunofluorescence and Western blot. Gene expression was analyzed with a quantitative real-time polymerase chain reaction. Results: Obtained particles were in the nano-range (average size around 50 nm) and had a spherical-like morphology. The typical hydrodynamic size was in the range 178-202 nm and Zeta potential equaled -9.51 mV. Mössbauer spectrum corresponds to the Fe+3 ions in tetrahedral (A) and Fe+3 and Fe+2 ions in octahedral (B) sites of Fe3O4. In vitro study revealed cytocompatibility and anti-inflammatory effects of fabricated nanoparticles. Furthermore, it was shown that nanoparticles combined with magnetic field exposure enhance osteogenic differentiation of MC3T3 cells by upregulation of RUNX-2 activity. Under the same experimental condition, nanoparticles and magnetic field decreased osteoclastogenesis of 4B12 by the induction of apoptosis through the mitochondrial-dependent pathway. Conclusion: Fe3O4 nanoparticles together with magnetic field can be applied for the fabrication of novel biomaterials for the treatment of bone disorders related to bone loss in which a balance between bone-forming and resorbing cells is disturbed.
Authors: Martin Lundqvist; Johannes Stigler; Giuliano Elia; Iseult Lynch; Tommy Cedervall; Kenneth A Dawson Journal: Proc Natl Acad Sci U S A Date: 2008-09-22 Impact factor: 11.205
Authors: Krzysztof Marycz; Michalina Alicka; Katarzyna Kornicka-Garbowska; Joanna Polnar; Anna Lis-Bartos; Rafał J Wiglusz; Michael Roecken; Jean-Marie Nedelec Journal: J Biomed Mater Res B Appl Biomater Date: 2019-09-12 Impact factor: 3.368
Authors: K Marycz; P Sobierajska; M Roecken; K Kornicka-Garbowska; M Kępska; R Idczak; J-M Nedelec; R J Wiglusz Journal: J Nanobiotechnology Date: 2020-02-18 Impact factor: 10.435