| Literature DB >> 36200826 |
Wei Lv1,2,3, Wei Jiang1,2, Hongmei Luo1,2, Qian Tong1,2, Xiaoyu Niu1,2, Xiao Liu1,2,4, Yang Miao1,2, Jingnan Wang1,2, Yiwen Guo1,2, Jianan Li1,2, Xizhen Zhan1,2, Yunqing Hou1,2, Yaxin Peng1,2, Jian Wang1,2,4, Shuhong Zhao1,2,3,5,6, Zaiyan Xu1,4, Bo Zuo1,2,3,5,6.
Abstract
Long noncoding RNAs (lncRNAs) play important roles in the spatial and temporal regulation of muscle development and regeneration. Nevertheless, the determination of their biological functions and mechanisms underlying muscle regeneration remains challenging. Here, we identified a lncRNA named lncMREF (lncRNA muscle regeneration enhancement factor) as a conserved positive regulator of muscle regeneration among mice, pigs and humans. Functional studies demonstrated that lncMREF, which is mainly expressed in differentiated muscle satellite cells, promotes myogenic differentiation and muscle regeneration. Mechanistically, lncMREF interacts with Smarca5 to promote chromatin accessibility when muscle satellite cells are activated and start to differentiate, thereby facilitating genomic binding of p300/CBP/H3K27ac to upregulate the expression of myogenic regulators, such as MyoD and cell differentiation. Our results unravel a novel temporal-specific epigenetic regulation during muscle regeneration and reveal that lncMREF/Smarca5-mediated epigenetic programming is responsible for muscle cell differentiation, which provides new insights into the regulatory mechanism of muscle regeneration.Entities:
Mesh：
Substances：
Year: 2022 PMID： 36200826 PMCID： PMC9561262 DOI： 10.1093/nar/gkac854
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 19.160