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Literature DB >> 3612958

Replicative intermediate of hepatitis B virus in transfected murine fibroblasts.

A Z Zelent, M A Sells, M Shvartsman, P M Price, G Acs.

Abstract

The NIH 3T3-derived cell line psi AM22b, which carries a defective Moloney murine leukemia virus, was transfected with a plasmid carrying the neo gene and two head-to-tail copies of the hepatitis B virus (HBV) genome positioned with opposing polarities. Both the two HBV dimers and the neo gene were located between two Moloney murine leukemia virus long terminal repeats. Poly(A)+ RNAs isolated from one clone that grew in the presence of G418 contained the two major classes of HBV-specific transcripts (3.5-kilobase pregenome and 2.1-kilobase mRNAs) in approximately equivalent amounts, which was reminiscent of the profiles of viral mRNAs from the livers of infected humans and chimpanzees.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA, Viral
RNA, Viral

Year: 1987 PMID： 3612958 PMCID： PMC255822

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

29 in total

1. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

Authors: A J Berk; P A Sharp
Journal: Cell Date: 1977-11 Impact factor: 41.582

2. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors: H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal: Biochemistry Date: 1977-10-18 Impact factor: 3.162

3. Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction.

Authors: T A Landers; H B Greenberg; W S Robinson
Journal: J Virol Date: 1977-08 Impact factor: 5.103

4. Amplification of expression of hepatitis B surface antigen in 3T3 cells cotransfected with a dominant-acting gene and cloned viral DNA.

Authors: J K Christman; M Gerber; P M Price; C Flordellis; J Edelman; G Acs
Journal: Proc Natl Acad Sci U S A Date: 1982-03 Impact factor: 11.205

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

6. Murine cells carrying integrated tandem genomes of hepatitis B virus DNA transcribe RNAs from endogenous promoters on both viral strands and express middle and major viral envelope proteins.

Authors: A Z Zelent; M A Sells; P M Price; A Mohamad; G Acs; J K Christman
Journal: J Virol Date: 1987-04 Impact factor: 5.103

Replicative intermediate of hepatitis B virus in transfected murine fibroblasts.

1. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.

2. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

3. Structure of hepatitis B Dane particle DNA and nature of the endogenous DNA polymerase reaction.

4. Amplification of expression of hepatitis B surface antigen in 3T3 cells cotransfected with a dominant-acting gene and cloned viral DNA.

5. Sequencing end-labeled DNA with base-specific chemical cleavages.

6. Murine cells carrying integrated tandem genomes of hepatitis B virus DNA transcribe RNAs from endogenous promoters on both viral strands and express middle and major viral envelope proteins.

7. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

8. Transformation of mammalian cells with an amplifiable dominant-acting gene.

9. Nucleotide sequence of the hepatitis B virus genome (subtype ayw) cloned in E. coli.

10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

1. Efficient duck hepatitis B virus production by an avian liver tumor cell line.

2. In vitro propagation of human hepatitis B virus in a rat hepatoma cell line.