Literature DB >> 3610675

An improved procedure for the X-ray microanalysis of acid phosphatase activity in lysosomes.

J B van Dort, J P Zeelen, W C de Bruijn.   

Abstract

The useful detection of acid phosphatase activity with cerium as a capturing agent is confirmed. By introducing a freeze step in combination with a preincubation, reliably localized, lysosomal precipitates are obtained and aspecific ones prevented. Short (t less than 1 h) postfixation with either OsO4 plus K4Fe (CN)6 or OsO4 plus aminotriazole, added to lysosomal cerium localization a high membrane contrast. The detection of cerium by X-ray microanalysis is improved by a better spectral separation of the osmium (M alpha) and cerium (L alpha) peaks.

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Year:  1987        PMID： 3610675     DOI： 10.1007/bf00518727

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  18 in total

1.  A new dynamic model system for the study of capture reactions for diffusable compounds in cytochemistry. III. Influence of the matrix composition on the lead phosphate precipitation process in acid phosphatase cytochemistry.

Authors:  A S De Jong; T J Hak; P Van Duijn; W T Daems
Journal:  Histochem J       Date:  1979-03

2.  Prevention of penetration hindrance in cerium-based glucose-6-phosphatase cytochemistry by freezing tissue in melting nitrogen.

Authors:  D Kalicharan; C E Hulstaert; M J Hardonk
Journal:  Histochemistry       Date:  1985

3.  Improved localization of intracellular sites of phosphatases using cerium and cell permeabilization.

Authors:  J M Robinson
Journal:  J Histochem Cytochem       Date:  1985-08       Impact factor: 2.479

4.  A new method for the investigation of the kinetics of the capture reaction in phosphatase cytochemistry. II. Theoretical and experimental study of phosphate diffusion from thin polyacrylamide films.

Authors:  C J Cornelisse; P van Duijn
Journal:  J Histochem Cytochem       Date:  1973-07       Impact factor: 2.479

5.  An ultrastructural characterization of the endothelial cell in the rat liver sinusoid under normal and various experimental conditions, as a contribution to the distinction between endothelial and Kupffer cells.

Authors:  E Wisse
Journal:  J Ultrastruct Res       Date:  1972-03

6.  Cytochemical demonstration of phosphatases in the rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation.

Authors:  C E Hulstaert; D Kalicharan; M J Hardonk
Journal:  Histochemistry       Date:  1983

7.  Application of Chelex standard beads in integrated morphometrical and X-ray microanalysis.

Authors:  W C de Bruijn; M I Cleton-Soeteman
Journal:  Scan Electron Microsc       Date:  1985

8.  False localization of acid phosphatase activity in the nuclear envelope and endoplasmic reticulum of peritoneal macrophages.

Authors:  E C Hoefsmit; I L Eestermans; C Korn; P Van Duijn
Journal:  Histochem J       Date:  1985-02

9.  X-ray microanalysis of aldehyde-fixed glycogen contrast-stained by OsVI . FeII and OsVI . RuIV complexes.

Authors:  W C de Bruijn; J M van Buitenen
Journal:  J Histochem Cytochem       Date:  1980-11       Impact factor: 2.479

10.  Cellular membrane contrast and contrast differentiation with osmium triazole and tetrazole complexes.

Authors:  W C de Bruijn; A A Memelink; J C Riemersma
Journal:  Histochem J       Date:  1984-01
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  3 in total

1.  Preservation of structure and cytochemical reactivity at the ultrastructural level, using microwave irradiation.

Authors:  J B Van Dort; W C De Bruijn; C T Schneijdenberg; M E Boon; L P Kok
Journal:  Histochem J       Date:  1988 Jun-Jul

2.  Ultrastructural electron probe X-ray microanalytical reaction product identification of three different enzymes in the same mouse resident peritoneal macrophage.

Authors:  J B van Dort; G A Ketelaars; W T Daems; W C de Bruijn
Journal:  Histochemistry       Date:  1989

3.  The cerium perhydroxide-diaminobenzidine (Ce-H2O2-DAB) procedure. New methods for light microscopic phosphatase histochemistry and immunohistochemistry.

Authors:  K J Halbhuber; R Gossrau; U Möller; C E Hulstaert; N Zimmermann; H Feuerstein
Journal:  Histochemistry       Date:  1988
  3 in total

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