Literature DB >> 6307935

Cytochemical demonstration of phosphatases in the rat liver by a cerium-based method in combination with osmium tetroxide and potassium ferrocyanide postfixation.

C E Hulstaert, D Kalicharan, M J Hardonk.   

Abstract

Acid phosphatase, alkaline phosphatase, glucose-6-phosphatase, Mg-activated adenosine triphosphatase and 5'nucleotidase were demonstrated in the rat liver using a cerium-based method. This method can be applied routinely and yields better results than the lead-based method. The tissue was postfixed in osmium tetroxide and potassium ferrocyanide which considerably enhances the membrane contrast in comparison with solely osmium tetroxide postfixation. This facilitates the precise localization of the reaction product.

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Year:  1983        PMID： 6307935     DOI： 10.1007/bf00491113

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  6 in total

1.  Histochemistry of hepatic phosphatases of a physiologic pH; with special reference to the demonstration of bile canaliculi.

Authors:  M WACHSTEIN; E MEISEL
Journal:  Am J Clin Pathol       Date:  1957-01       Impact factor: 2.493

2.  Glucose 6-phosphatase and thiamine pyrophosphatase activities in the jejunal epithelium of the mouse.

Authors:  J S Hugon; M Borgers; D Maestracci
Journal:  J Histochem Cytochem       Date:  1970-05       Impact factor: 2.479

3.  A direct lead method for the electron microscopic visualization of alkaline phosphatase activity.

Authors:  J Hugon; M Borgers
Journal:  J Histochem Cytochem       Date:  1966-05       Impact factor: 2.479

4.  A cytochemical method for the demonstration of 5'-nucleotidase in mouse peritoneal macrophages, with cerium ions used as trapping agent.

Authors:  J Blok; J J Onderwater; R de Water; L A Ginsel
Journal:  Histochemistry       Date:  1982

5.  X-ray microanalysis of aldehyde-fixed glycogen contrast-stained by OsVI . FeII and OsVI . RuIV complexes.

Authors:  W C de Bruijn; J M van Buitenen
Journal:  J Histochem Cytochem       Date:  1980-11       Impact factor: 2.479

6.  Localization of NADH oxidase on the surface of human polymorphonuclear leukocytes by a new cytochemical method.

Authors:  R T Briggs; D B Drath; M L Karnovsky; M J Karnovsky
Journal:  J Cell Biol       Date:  1975-12       Impact factor: 10.539
  6 in total
  35 in total

1.  Demonstration of glucose-6-phosphatase and peroxisomal catalase activity by ultrastructural cytochemistry in oval cells from livers of carcinogen-treated rats.

Authors:  F Plenat; L Braun; N Fausto
Journal:  Am J Pathol       Date:  1988-01       Impact factor: 4.307

2.  New, improved lanthanide-based methods for the ultrastructural localization of acid and alkaline phosphatase activity.

Authors:  K J Halbhuber; N Zimmermann; W Linss
Journal:  Histochemistry       Date:  1988

3.  Fine structure and cytochemical analysis of the intestinal wall along the body of adult female of Litomosoides chagasfilhoi (Nematoda: Filarioidea).

Authors:  A H A de Moraes Neto; G S P Cunha; T F Ferreira; S N de Carvalho; E V Guimarães; W de Souza
Journal:  Parasitol Res       Date:  2006-01-17       Impact factor: 2.289

4.  Survey of the occurrence of adenosine polyphosphatase in extracellular matrix of rat tissues. A cytochemical investigation.

Authors:  C E Hulstaert; D Kalicharan; K Poelstra; W W Bakker; M J Hardonk
Journal:  Histochemistry       Date:  1991

5.  The formation of asbestos bodies by mouse peritoneal macrophages. An in vitro study.

Authors:  H K Koerten; J D de Bruijn; W T Daems
Journal:  Am J Pathol       Date:  1990-07       Impact factor: 4.307

6.  Thiamine pyrophosphatase cytochemistry in rat endometrium during the oestrous cycle.

Authors:  L Staneva-Dobrovski
Journal:  Histochemistry       Date:  1994-08

7.  Demonstration of 5'-nucleotidase activity in unfixed cryostat sections of rat liver using a combined light- and electron-microscope procedure.

Authors:  J Song; K S Bosch; W Tigchelaar; R J Van Den Munckhof; J P Schellens; C J Van Noorden; W M Frederiks
Journal:  Histochem J       Date:  1995-11

8.  Light microscopical visualization of phosphatase activities demonstrated with the cerium method in resin sections of the kidney with the microwave oven.

Authors:  D Kalicharan; C E Hulstaert
Journal:  Histochemistry       Date:  1989

9.  Cytochemical demonstration of phosphatases in membrane-recycling structures of endodermal cells.

Authors:  H W Volk; P Kugler
Journal:  Histochemistry       Date:  1987

10.  Electron microscopic cytochemical localization of alpha-hydroxyacid oxidase in rat kidney cortex. Heterogeneous staining of peroxisomes.

Authors:  S Angermüller; C Leupold; K Zaar; H D Fahimi
Journal:  Histochemistry       Date:  1986
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