Ivan Sammir Aranda-Uribe1, Julian Ramírez-Bello2, Georgina Victoria-Acosta3, Felipe Muñoz-González2, Rosa Elda Barbosa-Cobos4, José Moreno5. 1. Department of Pharmacy, Division of Health Sciences, Universidad de Quintana Roo, Quintana Roo, Mexico. 2. Department of Endocrinology, Instituto Nacional de Cardiología, Mexico City, Mexico. 3. Direction of Research, Hospital Juárez de México, Mexico City, Mexico. 4. Service of Rheumatology, Hospital Juaréz de México, Mexico City, Mexico. 5. Direction of Research, Hospital Juárez de México, Mexico City, Mexico. jmoreno49@gmail.com.
Abstract
Rheumatoid arthritis (RA) is a chronic inflammatory disease of autoimmune origin with many associated genetic traits, including genes related to the control of inflammation. The A20 protein, encoded by the TNFAIP3 gene, is a negative regulator of NF-kB mediated inflammation. Several single nucleotide variants (SNVs) of TNFAIP3 are associated with susceptibility to RA in different ethnic groups, none of which has been evaluated in Mexican patients. OBJECTIVE: To examine the possible association of eight TNFAIP3 SNVs in Mexican patients with RA. MATERIALS: We studied 471 patients with RA and 500 controls, as well as eight TNFAIP3 SNVs: including, rs10499194C/T, rs6920220G/A, and rs2230926T/G, which have been associated with RA in European or Asian patients, in addition to rs373421182G/C, rs139054966T/G, rs5029924C/T, rs59693083A/G and rs61593413T/A, not previously examined in RA. All SNVs were evaluated by means of an allelic discrimination assay using TaqMan probes. RESULTS: The allelic and genotypic frequencies of all SNVs examined were similar between cases and controls, and none of them was associated with RA under the allelic, codominant, dominant, and recessive models, as well as in haplotype combinations. CONCLUSION: Our data indicate that TNFAIP3 SNVs evaluated herein are not risk factors for RA in Mexican subjects.
Rheumatoid arthritis (RA) is a chronic inflammatory disease of autoimmune origin with many associated genetic traits, including genes related to the control of inflammation. The A20 protein, encoded by the TNFAIP3 gene, is a negative regulator of NF-kB mediated inflammation. Several single nucleotide variants (SNVs) of TNFAIP3 are associated with susceptibility to RA in different ethnic groups, none of which has been evaluated in Mexican patients. OBJECTIVE: To examine the possible association of eight TNFAIP3 SNVs in Mexican patients with RA. MATERIALS: We studied 471 patients with RA and 500 controls, as well as eight TNFAIP3 SNVs: including, rs10499194C/T, rs6920220G/A, and rs2230926T/G, which have been associated with RA in European or Asian patients, in addition to rs373421182G/C, rs139054966T/G, rs5029924C/T, rs59693083A/G and rs61593413T/A, not previously examined in RA. All SNVs were evaluated by means of an allelic discrimination assay using TaqMan probes. RESULTS: The allelic and genotypic frequencies of all SNVs examined were similar between cases and controls, and none of them was associated with RA under the allelic, codominant, dominant, and recessive models, as well as in haplotype combinations. CONCLUSION: Our data indicate that TNFAIP3 SNVs evaluated herein are not risk factors for RA in Mexican subjects.
Authors: Stacy L Musone; Kimberly E Taylor; Timothy T Lu; Joanne Nititham; Ricardo C Ferreira; Ward Ortmann; Nataliya Shifrin; Michelle A Petri; M Ilyas Kamboh; Susan Manzi; Michael F Seldin; Peter K Gregersen; Timothy W Behrens; Averil Ma; Pui-Yan Kwok; Lindsey A Criswell Journal: Nat Genet Date: 2008-09 Impact factor: 38.330
Authors: Wendy Thomson; Anne Barton; Xiayi Ke; Steve Eyre; Anne Hinks; John Bowes; Rachelle Donn; Deborah Symmons; Samantha Hider; Ian N Bruce; Anthony G Wilson; Ioanna Marinou; Ann Morgan; Paul Emery; Angela Carter; Sophia Steer; Lynne Hocking; David M Reid; Paul Wordsworth; Pille Harrison; David Strachan; Jane Worthington Journal: Nat Genet Date: 2007-11-04 Impact factor: 38.330