Denis Rimashevskiy1, Franziska Schmidt2,3, Mike Barbeck4,5, Said Alkildani6,5, Armando Mandlule7, Milena Radenković8, Stevo Najman8,9, Sanja Stojanović8,9, Ole Jung6, Yanru Ren6,1, Baoyi Cai6,1, Oliver Görke7. 1. Department of Traumatology and Orthopedics, Peoples' Friendship University of Russia, Moscow, Russia. 2. Department of Prosthodontics, Geriatric Dentistry and Craniomandibular Disorders, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin, Germany. 3. Berlin Institute of Health, Berlin, Germany. 4. Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany; mike.barbeck@med.uni-rostock.de. 5. BerlinAnalytix GmbH, Berlin, Germany. 6. Clinic and Policlinic for Dermatology and Venereology, University Medical Center Rostock, Rostock, Germany. 7. Department of Ceramic Materials, Institute for Materials Science and Technologies, Technical University of Berlin, Berlin, Germany. 8. Department for Cell and Tissue Engineering, Faculty of Medicine, University of Niš, Niš, Serbia. 9. Department of Biology and Human Genetics, Faculty of Medicine, University of Niš, Niš, Serbia.
Abstract
BACKGROUND: Bioglass is a highly adoptable bone substitute material which can be combined with so-called therapeutic ions. However, knowledge is poor regarding the influence of therapeutic ions on immune reactions and associated bone healing. Thus, the aim of this work was to investigate the influence of strontium- and copper-doped bioglass on the induction of M1 and M2 macrophages, as well as vascularization. MATERIALS AND METHODS: Two types of alkali glass were produced based on ICIE16 bioglass via the melt-quench method with the addition of 5 wt% copper or strontium (ICIE16-Cu and ICIE16-Sr). Pure ICIE16 and 45S5 bioglass were used as control materials. The ion release and chemical composition of the bioglass were investigated, and an in vivo experiment was subcutaneously performed on Sprague-Dawley rats. RESULTS: Scanning electron microscopy revealed significant differences in the surface morphology of the bioglass materials. Energy dispersive X-ray spectroscopy confirmed the efficiency of the doping process by showing the ion-release kinetics. ICIE16-Cu exhibited a higher ion release than ICIE16-Sr. ICIE16-Cu induced low immune cell migration and triggered not only a low number of M1 and M2 macrophages but also of blood vessels. ICIE16-Sr induced higher numbers of M1 macrophages after 30 days. Both bioglass types induced numbers of M2 macrophages comparable with those found in the control groups. CONCLUSION: Bioglass doping with copper and strontium did not significantly influence the foreign body response nor vascularization of the implantation bed in vivo. However, all the studied bioglass materials seemed to be biocompatible.
BACKGROUND: Bioglass is a highly adoptable bone substitute material which can be combined with so-called therapeutic ions. However, knowledge is poor regarding the influence of therapeutic ions on immune reactions and associated bone healing. Thus, the aim of this work was to investigate the influence of strontium- and copper-doped bioglass on the induction of M1 and M2 macrophages, as well as vascularization. MATERIALS AND METHODS: Two types of alkali glass were produced based on ICIE16 bioglass via the melt-quench method with the addition of 5 wt% copper or strontium (ICIE16-Cu and ICIE16-Sr). Pure ICIE16 and 45S5 bioglass were used as control materials. The ion release and chemical composition of the bioglass were investigated, and an in vivo experiment was subcutaneously performed on Sprague-Dawley rats. RESULTS: Scanning electron microscopy revealed significant differences in the surface morphology of the bioglass materials. Energy dispersive X-ray spectroscopy confirmed the efficiency of the doping process by showing the ion-release kinetics. ICIE16-Cu exhibited a higher ion release than ICIE16-Sr. ICIE16-Cu induced low immune cell migration and triggered not only a low number of M1 and M2 macrophages but also of blood vessels. ICIE16-Sr induced higher numbers of M1 macrophages after 30 days. Both bioglass types induced numbers of M2 macrophages comparable with those found in the control groups. CONCLUSION: Bioglass doping with copper and strontium did not significantly influence the foreign body response nor vascularization of the implantation bed in vivo. However, all the studied bioglass materials seemed to be biocompatible.
Keywords:
45S5; Bioglass; DIN EN ISO 10993-6; ICIE16; bone tissue regeneration; copper doping; hydroxyapatite deposition; ion release; macrophages; strontium doping; vascularization
Authors: Roya Lari; Andrew J Fleetwood; Peter D Kitchener; Andrew D Cook; Durda Pavasovic; Paul J Hertzog; John A Hamilton Journal: Bone Date: 2006-10-18 Impact factor: 4.398
Authors: R Hernandez-Pando; Q L Bornstein; D Aguilar Leon; E H Orozco; V K Madrigal; E Martinez Cordero Journal: Immunology Date: 2000-07 Impact factor: 7.397