Literature DB >> 36066720

Live Cell Synthetic Histone Acetylation by Chemical Catalyst.

Shigehiro A Kawashima1, Motomu Kanai2.   

Abstract

Posttranslational modifications (PTMs) of histones, such as lysine acetylation and ubiquitination, regulate chromatin structure and gene expression. In living organisms, histone PTMs are catalyzed by histone-modifying enzymes. Here, we describe an entirely chemical method to introduce histone modifications in living cells without genetic manipulation. The chemical catalyst PEG-LANA-DSSMe activates a thioester acetyl donor, N,S-diacetylcysteamine (NAC-Ac), and promotes regioselective, synthetic histone acetylation at H2BK120 in living cells.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Acetylation; Catalyst; Histone

Mesh：

Substances：

Year:  2023        PMID： 36066720     DOI： 10.1007/978-1-0716-2433-3_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  11 in total

Review 1.  Histones: at the crossroads of peptide and protein chemistry.

Authors:  Manuel M Müller; Tom W Muir
Journal:  Chem Rev       Date:  2014-10-20       Impact factor: 60.622

2.  Visualizing the Dynamics of Inactive X Chromosomes in Living Cells Using Antibody-Based Fluorescent Probes.

Authors:  Yuko Sato; Timothy J Stasevich; Hiroshi Kimura
Journal:  Methods Mol Biol       Date:  2018

Review 3.  Leading approaches in synthetic epigenetics for novel therapeutic strategies.

Authors:  Kenzo Yamatsugu; Shigehiro A Kawashima; Motomu Kanai
Journal:  Curr Opin Chem Biol       Date:  2018-04-06       Impact factor: 8.822

4.  Synthetic Posttranslational Modifications: Chemical Catalyst-Driven Regioselective Histone Acylation of Native Chromatin.

Authors:  Yoshifumi Amamoto; Yuki Aoi; Nozomu Nagashima; Hiroki Suto; Daisuke Yoshidome; Yasuhiro Arimura; Akihisa Osakabe; Daiki Kato; Hitoshi Kurumizaka; Shigehiro A Kawashima; Kenzo Yamatsugu; Motomu Kanai
Journal:  J Am Chem Soc       Date:  2017-05-23       Impact factor: 15.419

5.  A chemical biology route to site-specific authentic protein modifications.

Authors:  Aerin Yang; Sura Ha; Jihye Ahn; Rira Kim; Sungyoon Kim; Younghoon Lee; Jaehoon Kim; Dieter Söll; Hee-Yoon Lee; Hee-Sung Park
Journal:  Science       Date:  2016-09-29       Impact factor: 47.728

6.  Discovering Targets of Non-enzymatic Acylation by Thioester Reactivity Profiling.

Authors:  Rhushikesh A Kulkarni; Andrew J Worth; Thomas T Zengeya; Jonathan H Shrimp; Julie M Garlick; Allison M Roberts; David C Montgomery; Carole Sourbier; Benjamin K Gibbs; Clementina Mesaros; Yien Che Tsai; Sudipto Das; King C Chan; Ming Zhou; Thorkell Andresson; Allan M Weissman; W Marston Linehan; Ian A Blair; Nathaniel W Snyder; Jordan L Meier
Journal:  Cell Chem Biol       Date:  2017-02-02       Impact factor: 8.116

7.  Chemical tagging and customizing of cellular chromatin states using ultrafast trans-splicing inteins.

Authors:  Yael David; Miquel Vila-Perelló; Shivam Verma; Tom W Muir
Journal:  Nat Chem       Date:  2015-04-06       Impact factor: 24.427

Review 8.  Chromatin deregulation in disease.

Authors:  Anne C Mirabella; Benjamin M Foster; Till Bartke
Journal:  Chromosoma       Date:  2015-07-19       Impact factor: 4.316

Review 9.  Writing, erasing and reading histone lysine methylations.

Authors:  Kwangbeom Hyun; Jongcheol Jeon; Kihyun Park; Jaehoon Kim
Journal:  Exp Mol Med       Date:  2017-04-28       Impact factor: 8.718
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