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Literature DB >> 3605649

Prolonged and simplified in vitro culture of explanted chick embryos.

Abstract

The method described in this paper allows explanted chick embryos to develop normally in vitro from the 2nd to the 5th day of incubation. The embryos are explanted with the aid of a ring of filter-paper and are fixed between two steel-rings dorsal side up for cultivation. The lower ring contains a mixture of yolk and albumen as culture medium. 40% of the explanted embryos reach the 5th day of development (stage 25, Hamburger and Hamilton). The maximum time of culture is 70 h after explantation.

Entities: Species

Mesh：

Substances：
Culture Media

Year: 1987 PMID： 3605649 DOI： 10.1007/bf00309751

Source DB: PubMed Journal: Anat Embryol (Berl) ISSN： 0340-2061

11 in total

1. A simple method for cultivating the early chick embryo in vitro.

Authors: K Palén; L Thörneby
Journal: Experientia Date: 1976-02-15

2. Development in vitro of the early chick blastoderm explanted on yolk and albumen extract saline-agar substrata.

Authors: N T SPRATT
Journal: J Exp Zool Date: 1947-12

3. A series of normal stages in the development of the chick embryo.

Authors: V HAMBURGER; H L HAMILTON
Journal: J Morphol Date: 1951-01 Impact factor: 1.804

4. A simple procedure for the long-term cultivation of chicken embryos.

Authors: R Auerbach; L Kubai; D Knighton; J Folkman
Journal: Dev Biol Date: 1974-12 Impact factor: 3.582

5. Description of a device facilitating the in vitro culture of chick embryo according to the new method.

Authors: W Seidl
Journal: Folia Morphol (Praha) Date: 1977

6. Angiogenesis induced by degradation products of hyaluronic acid.

Authors: D C West; I N Hampson; F Arnold; S Kumar
Journal: Science Date: 1985-06-14 Impact factor: 47.728

7. Cell movement in somite formation and development in the chick: inhibition of segmentation.

Authors: E A Chernoff; J W Lash
Journal: Dev Biol Date: 1981-10-30 Impact factor: 3.582

8. Effects of varying chamber construction and embryo pre-incubation age on survival and growth of chick embryos in shell-less culture.

Authors: B E Dunn; T P Fitzharris; B D Barnett
Journal: Anat Rec Date: 1981-01

Prolonged and simplified in vitro culture of explanted chick embryos.

1. A simple method for cultivating the early chick embryo in vitro.

2. Development in vitro of the early chick blastoderm explanted on yolk and albumen extract saline-agar substrata.

3. A series of normal stages in the development of the chick embryo.

4. A simple procedure for the long-term cultivation of chicken embryos.

5. Description of a device facilitating the in vitro culture of chick embryo according to the new method.

6. Angiogenesis induced by degradation products of hyaluronic acid.

7. Cell movement in somite formation and development in the chick: inhibition of segmentation.

8. Effects of varying chamber construction and embryo pre-incubation age on survival and growth of chick embryos in shell-less culture.

9. Mesodermal expansion after arrest of the edge in the area vasculosa of the chick.

10. A new class of steroids inhibits angiogenesis in the presence of heparin or a heparin fragment.

1. Fate mapping study of the endoderm of the 1.5-day-old chick embryo.

2. Edge cell migration in the extraembryonic mesoderm of the chick embryo. An experimental and morphological study.

3. The immediate fate of cells in the epithelial somite of the chick embryo.

4. New methods for chicken embryo manipulations.

5. Is extraembryonic angiogenesis in the chick embryo controlled by the endoderm? A morphology study.

6. Reciprocal repression between Sox3 and snail transcription factors defines embryonic territories at gastrulation.