| Literature DB >> 64360 |
Abstract
Catalytic properties of terminal riboadenylate transferase from Escherichia coli and the products of the enzymic reaction were investigated. The kinetic analysis revealed that the reaction obeys the sequential ordered bi-bi mechanism. The application of conditions elaborated in this study resulted in the synthesis of products of defined size and efficient primer utilization. The tRNA(rA)n obtained was a good template for the synthesis of complementary DNA with reverse transcriptase.Entities:
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Year: 1976 PMID: 64360 DOI: 10.1111/j.1432-1033.1976.tb11148.x
Source DB: PubMed Journal: Eur J Biochem ISSN: 0014-2956