Literature DB >> 35857225

E. coli Expression and Purification of Microbial and Viral Rhodopsins.

Taras Balandin1,2, Dmytro Volkov3,4, Alexey Alekseev5, Kirill Kovalev3,4,5,6,7, Dmitry Bratanov3,4, Valentin Gordeliy6.   

Abstract

Microbial rhodopsins have become an indispensable tool for neurobiology. Of thousands of identified microbial rhodopsins, a minute fraction has been studied so far and they have shown remarkable functional diversity suggesting more great promises that this large family holds. Effective production of recombinant microbial and viral rhodopsins is a prerequisite for the success of functional and structural studies of these proteins. Escherichia coli (E. coli) are suitable for high yield expression of many of microbial and viral rhodopsins and they facilitate rapid exploration of this large protein family.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Escherichia coli; Membrane protein; Production; Recombinant; Rhodopsin

Mesh：

Substances：

Year:  2022        PMID： 35857225     DOI： 10.1007/978-1-0716-2329-9_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Stable expression clones and auto-induction for protein production in E. coli.

Authors:  F William Studier
Journal:  Methods Mol Biol       Date:  2014

2.  Crystal structure of a light-driven sodium pump.

Authors:  Ivan Gushchin; Vitaly Shevchenko; Vitaly Polovinkin; Kirill Kovalev; Alexey Alekseev; Ekaterina Round; Valentin Borshchevskiy; Taras Balandin; Alexander Popov; Thomas Gensch; Christoph Fahlke; Christian Bamann; Dieter Willbold; Georg Büldt; Ernst Bamberg; Valentin Gordeliy
Journal:  Nat Struct Mol Biol       Date:  2015-04-06       Impact factor: 15.369

Review 3.  Biophysics of rhodopsins and optogenetics.

Authors:  Hideki Kandori
Journal:  Biophys Rev       Date:  2020-02-17

4.  Membrane Protein Production in Escherichia coli.

Authors:  Benjamin C McIlwain; Ali A Kermani
Journal:  Methods Mol Biol       Date:  2020

5.  An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin.

Authors:  Dmitry Bratanov; Taras Balandin; Ekaterina Round; Vitaly Shevchenko; Ivan Gushchin; Vitaly Polovinkin; Valentin Borshchevskiy; Valentin Gordeliy
Journal:  PLoS One       Date:  2015-06-05       Impact factor: 3.240

6.  Crystal structure of Escherichia coli-expressed Haloarcula marismortui bacteriorhodopsin I in the trimeric form.

Authors:  Vitaly Shevchenko; Ivan Gushchin; Vitaly Polovinkin; Ekaterina Round; Valentin Borshchevskiy; Petr Utrobin; Alexander Popov; Taras Balandin; Georg Büldt; Valentin Gordeliy
Journal:  PLoS One       Date:  2014-12-05       Impact factor: 3.240

7.  Inward H+ pump xenorhodopsin: Mechanism and alternative optogenetic approach.

Authors:  Vitaly Shevchenko; Thomas Mager; Kirill Kovalev; Vitaly Polovinkin; Alexey Alekseev; Josephine Juettner; Igor Chizhov; Christian Bamann; Charlotte Vavourakis; Rohit Ghai; Ivan Gushchin; Valentin Borshchevskiy; Andrey Rogachev; Igor Melnikov; Alexander Popov; Taras Balandin; Francisco Rodriguez-Valera; Dietmar J Manstein; Georg Bueldt; Ernst Bamberg; Valentin Gordeliy
Journal:  Sci Adv       Date:  2017-09-22       Impact factor: 14.136

8.  Overexpression of Different Types of Microbial Rhodopsins with a Highly Expressible Bacteriorhodopsin from Haloarcula marismortui as a Single Protein in E. coli.

Authors:  Cheng-Hong Tu; Hsiu-Ping Yi; Shiang-Yuan Hsieh; Hong-Syuan Lin; Chii-Shen Yang
Journal:  Sci Rep       Date:  2018-09-19       Impact factor: 4.379

Review 9.  Integration of optogenetics with complementary methodologies in systems neuroscience.

Authors:  Christina K Kim; Avishek Adhikari; Karl Deisseroth
Journal:  Nat Rev Neurosci       Date:  2017-03-17       Impact factor: 34.870

10.  Unique structure and function of viral rhodopsins.

Authors:  Dmitry Bratanov; Kirill Kovalev; Jan-Philipp Machtens; Roman Astashkin; Igor Chizhov; Dmytro Soloviov; Dmytro Volkov; Vitaly Polovinkin; Dmitrii Zabelskii; Thomas Mager; Ivan Gushchin; Tatyana Rokitskaya; Yuri Antonenko; Alexey Alekseev; Vitaly Shevchenko; Natalya Yutin; Riccardo Rosselli; Christian Baeken; Valentin Borshchevskiy; Gleb Bourenkov; Alexander Popov; Taras Balandin; Georg Büldt; Dietmar J Manstein; Francisco Rodriguez-Valera; Christoph Fahlke; Ernst Bamberg; Eugene Koonin; Valentin Gordeliy
Journal:  Nat Commun       Date:  2019-10-30       Impact factor: 14.919
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