| Literature DB >> 35796982 |
Philipp E Merkl1,2, Christopher Schächner1, Michael Pilsl1, Katrin Schwank1, Catharina Schmid1, Gernot Längst1, Philipp Milkereit3, Joachim Griesenbeck1, Herbert Tschochner4.
Abstract
In archaea and bacteria the major classes of RNAs are synthesized by one DNA-dependent RNA polymerase (RNAP). In contrast, most eukaryotes have three highly specialized RNAPs to transcribe the nuclear genome. RNAP I synthesizes almost exclusively ribosomal (r)RNA, RNAP II synthesizes mRNA as well as many noncoding RNAs involved in RNA processing or RNA silencing pathways and RNAP III synthesizes mainly tRNA and 5S rRNA. This review discusses functional differences of the three nuclear core RNAPs in the yeast S. cerevisiae with a particular focus on RNAP I transcription of nucleolar ribosomal (r)DNA chromatin.Entities:
Keywords: Chromatin; Gene expression; Nucleosomes; RNA polymerase I; RNA polymerase II; RNA polymerase III; Ribosomal RNA genes; Saccharomyces cerevisiae; Transcription; Transcription factors; Yeast
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Year: 2022 PMID： 35796982 DOI： 10.1007/978-1-0716-2501-9_4
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745