Amandeep Kaur1, Satvir Kaur Grewal2, Sarabjit Kaur1, Achla Sharma3, Puja Srivastava3, Monika Garg4, Shabir Hussain Wani5, Parveen Chhuneja1, Kuldeep Singh1,6, Satinder Kaur7. 1. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India. 2. Department of Biochemistry, Punjab Agricultural University, Ludhiana, India. 3. Department of Plant Breeding and Genetics, Punjab Agricultural University, Ludhiana, India. 4. Shere-e-Kashmir University of Agricultural Sciences and Technology, Srinagar, Kashmir, India. 5. National Agri-Food Biotechnology Institute, Mohali, India. 6. International Crops Research Institute for the Semi-Arid Tropics, Hyderabad, India. 7. School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana, India. satinder.biotech@pau.edu.
Abstract
BACKGROUND: Exponentially increasing population and everchanging climatic conditions are two major concerns for global food security. Early sowing in the second fortnight of October is an emerging trend with farmers in Indo Gangetic Plains to avoid yield losses from terminal heat stress. This also benefits the use of residual soil moisture of rice crop, conserving about one irrigation. But most of the available wheat cultivars are not well adapted to early-season sowing. METHODS AND RESULTS: Two in-house developed SHWs, syn14128 and syn14170, were screened for juvenile heat stress. Seedling length, biochemical parameters, and expression of amylase gene immediately after heat shock (HS) of 45 °C for 12 h and 20 h, and 24 h indicated significantly lower malondialdehyde, hydrogen peroxide, and higher free radical scavenging activities. Syn14170 reported higher total soluble sugar (TSS) under both HS periods, while syn14128 had a sustainable TSS content and amylase activity under HS as well as the recovery period. CONCLUSIONS: Both the SHWs had lower oxidative damage along with high free radical scavenging under heat stress. The higher expression of amy4 along with sustainable TSS after heat stress in syn14128 indicated it as a potential source of juvenile heat stress tolerance. Variable response of SHWs to different biochemical parameters under heat stress opens future perspectives to explore the enzymatic pathways underlying these responses.
BACKGROUND: Exponentially increasing population and everchanging climatic conditions are two major concerns for global food security. Early sowing in the second fortnight of October is an emerging trend with farmers in Indo Gangetic Plains to avoid yield losses from terminal heat stress. This also benefits the use of residual soil moisture of rice crop, conserving about one irrigation. But most of the available wheat cultivars are not well adapted to early-season sowing. METHODS AND RESULTS: Two in-house developed SHWs, syn14128 and syn14170, were screened for juvenile heat stress. Seedling length, biochemical parameters, and expression of amylase gene immediately after heat shock (HS) of 45 °C for 12 h and 20 h, and 24 h indicated significantly lower malondialdehyde, hydrogen peroxide, and higher free radical scavenging activities. Syn14170 reported higher total soluble sugar (TSS) under both HS periods, while syn14128 had a sustainable TSS content and amylase activity under HS as well as the recovery period. CONCLUSIONS: Both the SHWs had lower oxidative damage along with high free radical scavenging under heat stress. The higher expression of amy4 along with sustainable TSS after heat stress in syn14128 indicated it as a potential source of juvenile heat stress tolerance. Variable response of SHWs to different biochemical parameters under heat stress opens future perspectives to explore the enzymatic pathways underlying these responses.
Authors: Jafar Jafarzadeh; David Bonnett; Jean-Luc Jannink; Deniz Akdemir; Susanne Dreisigacker; Mark E Sorrells Journal: PLoS One Date: 2016-09-22 Impact factor: 3.240