Literature DB >> 35519

Combined use of strain construction and affinity chromatography in the rapid, high-yield purification of 6-phosphogluconate dehydrogenase from Escherichia coli.

R E Wolf, F M Shea.   

Abstract

A rapid, high-yield method for purification of 6-phosphogluconate dehydrogenase from Escherichia coli K-12 is described. Sonic extracts prepared from heat-induced cultures of strain RW184, doubly lysogenic for the specialized transducing bacteriophage lambdacI857St68h80dgndhis and bearing a deletion of the gene for glucose 6-phosphate dehydrogenase, contained levels of 6-phosphogluconate dehydrogenase 15- to 20-fold higher than cultures of wild-type cells. Affinity chromatography on blue dextran-Sepharose with batchwise elution with 1 mM nicotinamide adenine dinucleotide phosphate affected a further 10-fold purification. Enzyme prepared in this manner was homogeneous according to electrophoresis on sodium dodecyl sulfate-polyacrylamide gels and immunoelectrophoresis using antiserum directed against it. Fructose 1,6-diphosphate is an inhibitor of enzyme activity.

Entities:  

Mesh：

Substances：

Year:  1979        PMID： 35519      PMCID： PMC218254          DOI： 10.1128/jb.138.1.171-175.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  Medium for the differentiation of acid producing colonies of staphylococci.

Authors:  R Z KORMAN; D T BERMAN
Journal:  J Bacteriol       Date:  1958-10       Impact factor: 3.490

2.  In vitro synthesis of a constitutive enzyme of Escherichia coli, 6-phosphogluconate dehydrogenase.

Authors:  T Isturiz; R E Wolf
Journal:  Proc Natl Acad Sci U S A       Date:  1975-11       Impact factor: 11.205

3.  Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.

Authors:  S T Thompson; K H Cass; E Stellwagen
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Affinity chromatography of kinases and dehydrogenases on Sephadex and Sepharose dye derivatives.

Authors:  R L Easterday; I M Easterday
Journal:  Adv Exp Med Biol       Date:  1974       Impact factor: 2.622

6.  Isolation of specialized transducing bacteriophages for gluconate 6-phosphate dehydrogenase (gnd) of Escherichia coli.

Authors:  R E Wolf; D G Fraenkel
Journal:  J Bacteriol       Date:  1974-02       Impact factor: 3.490

7.  "Up-promoter" mutations of glucose 6-phosphate dehydrogenase in Escherichia coli.

Authors:  D G Fraenkel; A Parola
Journal:  J Mol Biol       Date:  1972-10-28       Impact factor: 5.469

8.  Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach.

Authors:  L D Ryan; C S Vestling
Journal:  Arch Biochem Biophys       Date:  1974-01       Impact factor: 4.013

9.  Binding of Cibacron blue F3GA to proteins containing the dinucleotide fold.

Authors:  S T Thompson; E Stellwagen
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

10.  Mechanism for regulating the distribution of glucose carbon between the Embden-Meyerhof and hexose-monophosphate pathways in Streptococcus faecalis.

Authors:  A T Brown; C L Wittenberger
Journal:  J Bacteriol       Date:  1971-05       Impact factor: 3.490
View more
  10 in total

1.  Growth-rate-dependent expression and cloning of gnd alleles from natural isolates of Escherichia coli.

Authors:  G J Barcak; R E Wolf
Journal:  J Bacteriol       Date:  1988-01       Impact factor: 3.490

2.  Molecular characterization of the Escherichia coli K-12 zwf gene encoding glucose 6-phosphate dehydrogenase.

Authors:  D L Rowley; R E Wolf
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

3.  Glucose 6-phosphate dehydrogenase is required for Salmonella typhimurium virulence and resistance to reactive oxygen and nitrogen intermediates.

Authors:  B E Lundberg; R E Wolf; M C Dinauer; Y Xu; F C Fang
Journal:  Infect Immun       Date:  1999-01       Impact factor: 3.441

4.  Genetic tagging, cloning, and DNA sequence of the Synechococcus sp. strain PCC 7942 gene (gnd) encoding 6-phosphogluconate dehydrogenase.

Authors:  S E Broedel; R E Wolf
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

5.  Different control circuits for growth rate-dependent regulation of 6-phosphogluconate dehydrogenase and protein components of the translational machinery in Escherichia coli.

Authors:  E E Farrish; H V Baker; R E Wolf
Journal:  J Bacteriol       Date:  1982-11       Impact factor: 3.490

6.  Growth rate-dependent regulation of 6-phosphogluconate dehydrogenase level in Escherichia coli K-12: beta-galactosidase expression in gnd-lac operon fusion strains.

Authors:  H V Baker; R E Wolf
Journal:  J Bacteriol       Date:  1983-02       Impact factor: 3.490

7.  Mapping of insertion mutations in gnd of Escherichia coli with deletions defining the ends of the gene.

Authors:  R E Wolf; J A Cool
Journal:  J Bacteriol       Date:  1980-03       Impact factor: 3.490

8.  Integration of specialized transducing bacteriophage lambda cI857 St68 h80 dgnd his by an unusual pathway promotes formation of deletions and generates a new translocatable element.

Authors:  R E Wolf
Journal:  J Bacteriol       Date:  1980-05       Impact factor: 3.490

9.  Molecular cloning, correlation of genetic and restriction maps, and determination of the direction of transcription of gnd of Escherichia coli.

Authors:  M S Nasoff; R E Wolf
Journal:  J Bacteriol       Date:  1980-08       Impact factor: 3.490

10.  Cloning and analysis of duplicated rfbM and rfbK genes involved in the formation of GDP-mannose in Escherichia coli O9:K30 and participation of rfb genes in the synthesis of the group I K30 capsular polysaccharide.

Authors:  P Jayaratne; D Bronner; P R MacLachlan; C Dodgson; N Kido; C Whitfield
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490
  10 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.