| Literature DB >> 164664 |
S T Thompson, K H Cass, E Stellwagen.
Abstract
A procedure is described to utilize blue dextran-Sepharose as an affinity chromatographic column specific for the super-secondary structure called the dinucleotide fold, which forms the binding sites for substrates and effectors on a wide range of proteins. The procedure can be used to identify proteins, either purified or in crude cellular extracts, that possess the dinucleotide fold and to significantly improve the purification procedures for those proteins that possess the fold.Entities:
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Year: 1975 PMID: 164664 PMCID: PMC432376 DOI: 10.1073/pnas.72.2.669
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205