Literature DB >> 3549703

Cloning and expression in Escherichia coli of a cellulase gene from Ruminococcus flavefaciens.

M E Barros, J A Thomson.   

Abstract

An endoglucanase gene of Ruminococcus flavefaciens FD1 was cloned on the vector pEcoR251 to form the recombinant plasmid pMEB200. The cloned endoglucanase gene showed carboxymethylcellulase enzyme activity but no degradation of Avicel (FMC Corp., Philadelphia, Pa.) or filter paper. Carboxymethylcellulase activity was found during the late-exponential-growth phase and accumulated in the periplasmic fraction. Enzyme production was not subject to catabolite repression by glucose.

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Year:  1987        PMID： 3549703      PMCID： PMC212014          DOI： 10.1128/jb.169.4.1760-1762.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  10 in total

1.  Properties and mode of action of cellulases.

Authors:  T M Wood
Journal:  Biotechnol Bioeng Symp       Date:  1975

2.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

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4.  Microiodometric determination of beta-lactamase activity.

Authors:  R B Sykes; K Nordström
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5.  Regulation of cellulase from Ruminococcus.

Authors:  M C Fusee; J M Leatherwood
Journal:  Can J Microbiol       Date:  1972-03       Impact factor: 2.419

6.  Rapid and efficient cosmid cloning.

Authors:  D Ish-Horowicz; J F Burke
Journal:  Nucleic Acids Res       Date:  1981-07-10       Impact factor: 16.971

7.  Simple, rapid, and quantitative release of periplasmic proteins by chloroform.

Authors:  G F Ames; C Prody; S Kustu
Journal:  J Bacteriol       Date:  1984-12       Impact factor: 3.490

8.  Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria.

Authors:  D R Caldwell; M P Bryant
Journal:  Appl Microbiol       Date:  1966-09

9.  Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.

Authors:  R M Teather; P J Wood
Journal:  Appl Environ Microbiol       Date:  1982-04       Impact factor: 4.792

10.  Effect of soluble carbohydrates on digestion of cellulose by pure cultures of rumen bacteria.

Authors:  P Hiltner; B A Dehority
Journal:  Appl Environ Microbiol       Date:  1983-09       Impact factor: 4.792
  10 in total
  14 in total

1.  Cloning, sequencing, and expression of a Eubacterium cellulosolvens 5 gene encoding an endoglucanase (Cel5A) with novel carbohydrate-binding modules, and properties of Cel5A.

Authors:  Kazutoyo Yoda; Atsushi Toyoda; Yoshihiro Mukoyama; Yutaka Nakamura; Hajime Minato
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

2.  Inhibitory Effects of Methylcellulose on Cellulose Degradation by Ruminococcus flavefaciens.

Authors:  M A Rasmussen; R B Hespell; B A White; R J Bothast
Journal:  Appl Environ Microbiol       Date:  1988-04       Impact factor: 4.792

3.  Molecular Cloning and Expression of Cellulase Genes from Ruminococcus albus 8 in Escherichia coli Bacteriophage lambda.

Authors:  G T Howard; B A White
Journal:  Appl Environ Microbiol       Date:  1988-07       Impact factor: 4.792

4.  Molecular cloning of genes from Ruminococcus flavefaciens encoding xylanase and beta(1-3,1-4)glucanase activities.

Authors:  H J Flint; C A McPherson; J Bisset
Journal:  Appl Environ Microbiol       Date:  1989-05       Impact factor: 4.792

5.  Cloning of an endo-1,4-beta-D-glucanase gene from Clostridium josui and its expression in Escherichia coli.

Authors:  K Ohmiya; T Fujino; J Sukhumavasi; S Shimizu
Journal:  Appl Environ Microbiol       Date:  1989-09       Impact factor: 4.792

6.  Assessment of the endo-1,4-beta-glucanase components of Ruminococcus flavefaciens FD-1.

Authors:  K C Doerner; B A White
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

7.  Sequencing and expression of a cellodextrinase (ced1) gene from Butyrivibrio fibrisolvens H17c cloned in Escherichia coli.

Authors:  E Berger; W A Jones; D T Jones; D R Woods
Journal:  Mol Gen Genet       Date:  1990-09

8.  Nucleotide sequence of the celA gene encoding a cellodextrinase of Ruminococcus flavefaciens FD-1.

Authors:  W Y Wang; J A Thomson
Journal:  Mol Gen Genet       Date:  1990-07

9.  Partial characterization of a DNA restriction endonuclease from Ruminococcus flavefaciens FD-1 and its inhibition by site-specific adenine methylation.

Authors:  M Morrison; R I Mackie; B A White
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792

10.  Molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans.

Authors:  S D Baird; D A Johnson; V L Seligy
Journal:  J Bacteriol       Date:  1990-03       Impact factor: 3.490
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