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Literature DB >> 6501229

Simple, rapid, and quantitative release of periplasmic proteins by chloroform.

Abstract

We introduce a method by which periplasmic proteins can be released rapidly, simply, and quantitatively by treating cells with chloroform. All the amino acid-binding proteins tested maintained their activity during chloroform treatment. This method makes practical the analysis of the periplasmic protein complement of a large number of strains.

Entities: Chemical

Mesh：

Substances：

Year: 1984 PMID： 6501229 PMCID： PMC215841 DOI： 10.1128/jb.160.3.1181-1183.1984

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

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Authors: P H O'Farrell
Journal: J Biol Chem Date: 1975-05-25 Impact factor: 5.157

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Authors: J B Stock; B Rauch; S Roseman
Journal: J Biol Chem Date: 1977-11-10 Impact factor: 5.157

3. Quantitative assay of the binding of small molecules to protein: comparison of dialysis and membrane filter assays.

Authors: J E Lever
Journal: Anal Biochem Date: 1972-11 Impact factor: 3.365

4. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.

Authors: G F Ames
Journal: J Biol Chem Date: 1974-01-25 Impact factor: 5.157

5. Multiple molecular forms of glutamine synthetase produced by enzyme catalyzed adenylation and deadenylylation reactions.

Authors: E R Stadtman; A Ginsburg; J E Ciardi; J Yeh; S B Hennig; B M Shapiro
Journal: Adv Enzyme Regul Date: 1970

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. The histidine-binding protein J, a histidine transport component, has two different functional sites.

Authors: S G Kustu; G F Ames
Journal: J Biol Chem Date: 1974-11-10 Impact factor: 5.157

8. Mutations affecting glutamine synthetase activity in Salmonella typhimurium.

Authors: S G Kustu; K McKereghan
Journal: J Bacteriol Date: 1975-06 Impact factor: 3.490

9. Nitrogen control of Salmonella typhimurium: co-regulation of synthesis of glutamine synthetase and amino acid transport systems.

Authors: S G Kustu; N C McFarland; S P Hui; B Esmon; G F Ames
Journal: J Bacteriol Date: 1979-04 Impact factor: 3.490

10. "In vivo" detection of L-arabinose-binding protein, CRM-negative mutants.

Authors: R W Hogg
Journal: J Bacteriol Date: 1971-02 Impact factor: 3.490

104 in total

1. Optical determination of glutamine using a genetically engineered protein.

Authors: J D Dattelbaum; J R Lakowicz
Journal: Anal Biochem Date: 2001-04-01 Impact factor: 3.365

2. Changes in fine structure and polypeptide pattern during development of Holospora obtusa, a bacterium infecting the macronucleus of Paramecium caudatum.

Authors: H D Görtz; S Lellig; O Miosga; M Wiemann
Journal: J Bacteriol Date: 1990-10 Impact factor: 3.490

3. Periplasmic superoxide dismutases in Aquaspirillum magnetotacticum.

Authors: K A Short; R P Blakemore
Journal: Arch Microbiol Date: 1989 Impact factor: 2.552

4. Cloning and expression of a tetracycline resistance determinant from Campylobacter jejuni in Escherichia coli.

Authors: F C Tenover; D J LeBlanc; P Elvrum
Journal: Antimicrob Agents Chemother Date: 1987-09 Impact factor: 5.191

5. A mutation in Flavobacterium psychrophilum tlpB inhibits gliding motility and induces biofilm formation.

Authors: B Alvarez; P Secades; M Prieto; M J McBride; J A Guijarro
Journal: Appl Environ Microbiol Date: 2006-06 Impact factor: 4.792

6. Freeze-Thawing of Aquaspirillum magnetotacticum Cells Selectively Releases Periplasmic Proteins.

Authors: L C Paoletti; K A Short; N Blakemore; R P Blakemore
Journal: Appl Environ Microbiol Date: 1987-10 Impact factor: 4.792

7. Biotinylation in vivo as a sensitive indicator of protein secretion and membrane protein insertion.

Authors: G Jander; J E Cronan; J Beckwith
Journal: J Bacteriol Date: 1996-06 Impact factor: 3.490

8. The "intracellular" poly(3-hydroxybutyrate) (PHB) depolymerase of Rhodospirillum rubrum is a periplasm-located protein with specificity for native PHB and with structural similarity to extracellular PHB depolymerases.

Authors: René Handrick; Simone Reinhardt; Philipp Kimmig; Dieter Jendrossek
Journal: J Bacteriol Date: 2004-11 Impact factor: 3.490

9. In vivo labeling of Escherichia coli cell envelope proteins with N-hydroxysuccinimide esters of biotin.

Authors: J A Bradburne; P Godfrey; J H Choi; J N Mathis
Journal: Appl Environ Microbiol Date: 1993-03 Impact factor: 4.792

10. alpha-Galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization.

Authors: D J Gage; S R Long
Journal: J Bacteriol Date: 1998-11 Impact factor: 3.490