Literature DB >> 3536958

Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins.

R L Last, J L Woolford.   

Abstract

Temperature-sensitive mutations in the RNA2 through RNA11 genes of yeast prevent the processing of nuclear pre-mRNAs. We have raised antisera that detect the RNA2 and RNA3 proteins in immunoblots of extracts of yeast containing high copy number RNA2 and RNA3 plasmids. Subcellular fractionation of yeast cells that overproduce the RNA2 and RNA3 proteins has revealed that these proteins are enriched in nuclear fractions. Indirect immunofluorescence results have indicated that these proteins are localized in yeast nuclei. These localization results are consistent with the fact that these genes have a role in processing yeast pre-mRNA.

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Year:  1986        PMID： 3536958      PMCID： PMC2114587          DOI： 10.1083/jcb.103.6.2103

Source DB:  PubMed          Journal:  J Cell Biol        ISSN： 0021-9525            Impact factor:   10.539


  45 in total

1.  The use of fluorescent DNA-binding agent for detecting and separating yeast mitochondrial DNA.

Authors:  D H Williamson; D J Fennell
Journal:  Methods Cell Biol       Date:  1975       Impact factor: 1.441

2.  A new method for sequencing DNA.

Authors:  A M Maxam; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1977-02       Impact factor: 11.205

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Labeling of proteins with beta-galactosidase by gene fusion. Identification of a cytoplasmic membrane component of the Escherichia coli maltose transport system.

Authors:  H A Shuman; T J Silhavy; J R Beckwith
Journal:  J Biol Chem       Date:  1980-01-10       Impact factor: 5.157

5.  Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.

Authors:  D Gallwitz; R Seidel
Journal:  Nucleic Acids Res       Date:  1980-03-11       Impact factor: 16.971

6.  Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.

Authors:  R Ng; J Abelson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-07       Impact factor: 11.205

7.  The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast.

Authors:  M Rosbash; P K Harris; J L Woolford; J L Teem
Journal:  Cell       Date:  1981-06       Impact factor: 41.582

8.  Yeast mRNA splicing in vitro.

Authors:  R J Lin; A J Newman; S C Cheng; J Abelson
Journal:  J Biol Chem       Date:  1985-11-25       Impact factor: 5.157

9.  Macromolecule synthesis in temperature-sensitive mutants of yeast.

Authors:  L H Hartwell
Journal:  J Bacteriol       Date:  1967-05       Impact factor: 3.490

10.  The genes for fifteen ribosomal proteins of Saccharomyces cerevisiae.

Authors:  H M Fried; N J Pearson; C H Kim; J R Warner
Journal:  J Biol Chem       Date:  1981-10-10       Impact factor: 5.157
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  16 in total

1.  Genetic studies of the PRP11 gene of Saccharomyces cerevisiae.

Authors:  K Schappert; J D Friesen
Journal:  Mol Gen Genet       Date:  1991-04

2.  U4 small nuclear RNA dissociates from a yeast spliceosome and does not participate in the subsequent splicing reaction.

Authors:  S L Yean; R J Lin
Journal:  Mol Cell Biol       Date:  1991-11       Impact factor: 4.272

3.  Identification of nuclear proteins that specifically bind to RNAs containing 5' splice sites.

Authors:  D T Stolow; S M Berget
Journal:  Proc Natl Acad Sci U S A       Date:  1991-01-15       Impact factor: 11.205

4.  Nuclear localization of Semliki Forest virus-specific nonstructural protein nsP2.

Authors:  J Peränen; M Rikkonen; P Liljeström; L Kääriäinen
Journal:  J Virol       Date:  1990-05       Impact factor: 5.103

5.  The yeast PRP2 protein, a putative RNA-dependent ATPase, shares extensive sequence homology with two other pre-mRNA splicing factors.

Authors:  J H Chen; R J Lin
Journal:  Nucleic Acids Res       Date:  1990-11-11       Impact factor: 16.971

6.  Differential distribution of factors involved in pre-mRNA processing in the yeast cell nucleus.

Authors:  J A Potashkin; R J Derby; D L Spector
Journal:  Mol Cell Biol       Date:  1990-07       Impact factor: 4.272

7.  PRP18, a protein required for the second reaction in pre-mRNA splicing.

Authors:  U Vijayraghavan; J Abelson
Journal:  Mol Cell Biol       Date:  1990-01       Impact factor: 4.272

8.  RNA11 protein is associated with the yeast spliceosome and is localized in the periphery of the cell nucleus.

Authors:  T H Chang; M W Clark; A J Lustig; M E Cusick; J Abelson
Journal:  Mol Cell Biol       Date:  1988-06       Impact factor: 4.272

9.  Interactions of PRP2 protein with pre-mRNA splicing complexes in Saccharomyces cerevisiae.

Authors:  D S King; J D Beggs
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

10.  Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing.

Authors:  S P Jackson; M Lossky; J D Beggs
Journal:  Mol Cell Biol       Date:  1988-03       Impact factor: 4.272
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