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Literature DB >> 7003553

Molecular cloning of the actin gene from yeast Saccharomyces cerevisiae.

Abstract

Two overlapping DNA fragments from yeast Saccharomyces cerevisiae containing the actin gene have been inserted into pBR322 and cloned in E.coli. Clones were identified by hybridization to complementary RNA from a plasmid containing a copy of Dictyostelium actin mRNA. One recombinant plasmid obtained (pYA102) contains a 3.93-kb Hindlll fragment, the other (pYA208) a 5.1-kb Pstl fragment, both share a common 2.2-kb fragment harboring part of the actin gene. Cloned yeast actin DNA was identified by R-loop formation and translation of the hybridized actin mRNA and by DNA sequence analysis. Cytoplasmic actin mRNA has been estimated to be about 1250 nucleotides long. There is only one type of the actin gene in S.cerevisiae.

Entities: Gene Species

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Year: 1980 PMID： 7003553 PMCID： PMC323972 DOI： 10.1093/nar/8.5.1043

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

30 in total

1. Biochemistry of actomyosin-dependent cell motility (a review).

Authors: E D Korn
Journal: Proc Natl Acad Sci U S A Date: 1978-02 Impact factor: 11.205

2. Mammalian cytoplasmic actins are the products of at least two genes and differ in primary structure in at least 25 identified positions from skeletal muscle actins.

Authors: J Vandekerckhove; K Weber
Journal: Proc Natl Acad Sci U S A Date: 1978-03 Impact factor: 11.205

3. Unique arrangement of coding sequences for 5 S, 5.8 S, 18 S and 25 S ribosomal RNA in Saccharomyces cerevisiae as determined by R-loop and hybridization analysis.

Authors: P Philippsen; M Thomas; R A Kramer; R W Davis
Journal: J Mol Biol Date: 1978-08-15 Impact factor: 5.469

4. Screening of cloned recombinant DNA in bacteria by in situ colony hybridization.

Authors: B Cami; P Kourilsky
Journal: Nucleic Acids Res Date: 1978-07 Impact factor: 16.971

5. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors: J C Alwine; D J Kemp; G R Stark
Journal: Proc Natl Acad Sci U S A Date: 1977-12 Impact factor: 11.205

6. Actins from mammals, bird, fish and slime mold characterized by isoelectric focusing in polyacrylamide gels.

Authors: K Zechel; K Weber
Journal: Eur J Biochem Date: 1978-08-15

7. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.

Authors: G K McMaster; G G Carmichael
Journal: Proc Natl Acad Sci U S A Date: 1977-11 Impact factor: 11.205

8. Identification and analysis of Dictyostelium actin genes, a family of moderately repeated genes.

Authors: K L Kindle; R A Firtel
Journal: Cell Date: 1978-11 Impact factor: 41.582

9. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors: F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal: Gene Date: 1977 Impact factor: 3.688

10. Partial amino acid sequence of brain actin and its homology with muscle actin.

Authors: R C Lu; M Elzinga
Journal: Biochemistry Date: 1977-12-27 Impact factor: 3.162

50 in total

1. Insect muscle actins differ distinctly from invertebrate and vertebrate cytoplasmic actins.

Authors: N Mounier; M Gouy; D Mouchiroud; J C Prudhomme
Journal: J Mol Evol Date: 1992-05 Impact factor: 2.395

2. F-actin homeostasis through transcriptional regulation and proteasome-mediated proteolysis.

Authors: Masayuki Onishi; Kresti Pecani; Taylor Jones; John R Pringle; Frederick R Cross
Journal: Proc Natl Acad Sci U S A Date: 2018-06-25 Impact factor: 11.205

3. Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.

Authors: F J Klinz; D Gallwitz
Journal: Nucleic Acids Res Date: 1985-06-11 Impact factor: 16.971

4. Construction of a yeast actin gene intron deletion mutant that is defective in splicing and leads to the accumulation of precursor RNA in transformed yeast cells.

Authors: D Gallwitz
Journal: Proc Natl Acad Sci U S A Date: 1982-06 Impact factor: 11.205

5. A new yeast gene with a myosin-like heptad repeat structure.

Authors: R Kölling; T Nguyen; E Y Chen; D Botstein
Journal: Mol Gen Genet Date: 1993-03

6. Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.

Authors: D Shortle; P Novick; D Botstein
Journal: Proc Natl Acad Sci U S A Date: 1984-08 Impact factor: 11.205

7. Expression of a recoded nuclear gene inserted into yeast mitochondrial DNA is limited by mRNA-specific translational activation.

Authors: D F Steele; C A Butler; T D Fox
Journal: Proc Natl Acad Sci U S A Date: 1996-05-28 Impact factor: 11.205

8. Isolation and characterization of the RNA2, RNA3, and RNA11 genes of Saccharomyces cerevisiae.

Authors: R L Last; J B Stavenhagen; J L Woolford
Journal: Mol Cell Biol Date: 1984-11 Impact factor: 4.272

9. AAR2, a gene for splicing pre-mRNA of the MATa1 cistron in cell type control of Saccharomyces cerevisiae.

Authors: N Nakazawa; S Harashima; Y Oshima
Journal: Mol Cell Biol Date: 1991-11 Impact factor: 4.272

10. Actin structure and function: roles in mitochondrial organization and morphogenesis in budding yeast and identification of the phalloidin-binding site.

Authors: D G Drubin; H D Jones; K F Wertman
Journal: Mol Biol Cell Date: 1993-12 Impact factor: 4.138