| Literature DB >> 2997224 |
R J Lin, A J Newman, S C Cheng, J Abelson.
Abstract
Synthetic actin and CYH2 pre-mRNAs containing a single intron are accurately spliced in a soluble whole cell extract of yeast. Splicing in vitro requires ATP. The excised intron is released as a lariat in which an RNA branch connects the 5' end of the molecule to the last A in the "intron conserved sequence" UACUAAC. Two other discrete RNA species produced during splicing in vitro may represent reaction intermediates: free, linear exon 1 and a form of the intron lariat extending beyond the 3' splice site to include exon 2. Both lariat forms correspond to molecules previously shown to be produced during yeast pre-mRNA splicing in vivo.Entities:
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Year: 1985 PMID： 2997224
Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157