| Literature DB >> 35294751 |
Srilatha Sakamuru1, Jinghua Zhao1, Matias S Attene-Ramos2, Menghang Xia3.
Abstract
Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2, HepaRG, and AC16 cells. A homogenous cell-based MMP assay has been optimized and performed in a 1536-well plate format, which can be used to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.Entities:
Keywords: 1536-well plate; Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone (FCCP); Mitochondrial membrane potential (MMP); Mitochondrial membrane potential indicator (m-MPI); Mitochondrial toxicity
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Year: 2022 PMID： 35294751 DOI： 10.1007/978-1-0716-2213-1_2
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745