Literature DB >> 3526349

Molecular analysis of the DNA sequences involved in the transcriptional regulation of the phosphate-repressible acid phosphatase gene (PHO5) of Saccharomyces cerevisiae.

L W Bergman, D C McClinton, S L Madden, L H Preis.   

Abstract

The expression of the PHO5 gene of Saccharomyces cerevisiae is transcriptionally regulated in response to the level of inorganic phosphate present in the growth medium. We have identified, by DNA deletion analysis, the sequences (upstream activator sequences) that mediate this response. The sequence 5' CTGCACAAATG 3' is present in two copies located within a 60-base-pair region. The presence of a single copy of the sequence is sufficient for the phosphate-mediated transcriptional response. In addition, a DNA fragment that contains two copies of this sequence will act to repress transcription of a CYC1-lacZ fusion when placed either upstream or downstream of the CYC1 activator sequence.

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Year:  1986        PMID： 3526349      PMCID： PMC386440          DOI： 10.1073/pnas.83.16.6070

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  26 in total

1.  Organization of the GAL1-GAL10 intergenic control region chromatin.

Authors:  D Lohr
Journal:  Nucleic Acids Res       Date:  1984-11-26       Impact factor: 16.971

2.  A positive regulatory site and a negative regulatory site control the expression of the Saccharomyces cerevisiae CYC7 gene.

Authors:  C F Wright; R S Zitomer
Journal:  Mol Cell Biol       Date:  1984-10       Impact factor: 4.272

3.  Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae.

Authors:  G Lucchini; A G Hinnebusch; C Chen; G R Fink
Journal:  Mol Cell Biol       Date:  1984-07       Impact factor: 4.272

4.  The chromatin structure of an actively expressed, single copy yeast gene.

Authors:  D Lohr
Journal:  Nucleic Acids Res       Date:  1983-10-11       Impact factor: 16.971

5.  A bacterial repressor protein or a yeast transcriptional terminator can block upstream activation of a yeast gene.

Authors:  R Brent; M Ptashne
Journal:  Nature       Date:  1984 Dec 13-19       Impact factor: 49.962

6.  Negative regulation of STE6 gene expression by the alpha 2 product of Saccharomyces cerevisiae.

Authors:  K L Wilson; I Herskowitz
Journal:  Mol Cell Biol       Date:  1984-11       Impact factor: 4.272

7.  A GAL10-CYC1 hybrid yeast promoter identifies the GAL4 regulatory region as an upstream site.

Authors:  L Guarente; R R Yocum; P Gifford
Journal:  Proc Natl Acad Sci U S A       Date:  1982-12       Impact factor: 11.205

8.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

9.  Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae.

Authors:  L Guarente; B Lalonde; P Gifford; E Alani
Journal:  Cell       Date:  1984-02       Impact factor: 41.582

10.  Modulation of chromatin structure associated with derepression of the acid phosphatase gene of Saccharomyces cerevisiae.

Authors:  L W Bergman; R A Kramer
Journal:  J Biol Chem       Date:  1983-06-10       Impact factor: 5.157
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  10 in total

1.  Molecular and expression analysis of the negative regulators involved in the transcriptional regulation of acid phosphatase production in Saccharomyces cerevisiae.

Authors:  S L Madden; D L Johnson; L W Bergman
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

2.  Cloning, sequencing, and characterization of the principal acid phosphatase, the phoC+ product, from Zymomonas mobilis.

Authors:  J L Pond; C K Eddy; K F Mackenzie; T Conway; D J Borecky; L O Ingram
Journal:  J Bacteriol       Date:  1989-02       Impact factor: 3.490

3.  Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85.

Authors:  Y Uesono; K Tanaka; A Toh-e
Journal:  Nucleic Acids Res       Date:  1987-12-23       Impact factor: 16.971

4.  Mode of expression of the positive regulatory genes PHO2 and PHO4 of the phosphatase regulon in Saccharomyces cerevisiae.

Authors:  K Yoshida; Z Kuromitsu; N Ogawa; Y Oshima
Journal:  Mol Gen Genet       Date:  1989-05

5.  The two positively acting regulatory proteins PHO2 and PHO4 physically interact with PHO5 upstream activation regions.

Authors:  K Vogel; W Hörz; A Hinnen
Journal:  Mol Cell Biol       Date:  1989-05       Impact factor: 4.272

6.  The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.

Authors:  H Rudolph; A Hinnen
Journal:  Proc Natl Acad Sci U S A       Date:  1987-03       Impact factor: 11.205

7.  Structure and expression of the PHO80 gene of Saccharomyces cerevisiae.

Authors:  S L Madden; C L Creasy; V Srinivas; W Fawcett; L W Bergman
Journal:  Nucleic Acids Res       Date:  1988-03-25       Impact factor: 16.971

8.  Complementation of Saccharomyces cerevisiae acid phosphatase mutation by a genomic sequence from the yeast Yarrowia lipolytica identifies a new phosphatase.

Authors:  B Y Tréton; M T Le Dall; C M Gaillardin
Journal:  Curr Genet       Date:  1992-11       Impact factor: 3.886

9.  Heterologous protein secretion directed by a repressible acid phosphatase system of Kluyveromyces lactis: characterization of upstream region-activating sequences in the KIPHO5 gene.

Authors:  E Fermiñán; A Domínguez
Journal:  Appl Environ Microbiol       Date:  1998-07       Impact factor: 4.792

10.  Specific cis-acting sequence for PHO8 expression interacts with PHO4 protein, a positive regulatory factor, in Saccharomyces cerevisiae.

Authors:  N Hayashi; Y Oshima
Journal:  Mol Cell Biol       Date:  1991-02       Impact factor: 4.272
  10 in total

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