Literature DB >> 3320965

Negative regulators of the PHO system in Saccharomyces cerevisiae: isolation and structural characterization of PHO85.

Y Uesono1, K Tanaka, A Toh-e.   

Abstract

One of the negative regulators of the PHO system of Saccharomyces cerevisiae, PHO85, has been isolated by transformation and complementation of a pho85 strain. The complementing activity was delimited within a 1258 bp DNA segment and this region has been sequenced. The largest open reading frame found in this region can encode a protein of 302 amino acid residues. A pho85 mutant resulted from disruption of the chromosomal counterpart of the open reading frame described above. Therefore, we concluded that the gene we have cloned is PHO85. This result also indicates that PHO85 is nonessential. Northern analysis revealed that the size of the PHO85 message is 1.1 kb. No similarity was found between the putative amino acid sequences of two negative regulators, the PHO80 and PHO85 proteins.

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Year:  1987        PMID： 3320965      PMCID： PMC339945          DOI： 10.1093/nar/15.24.10299

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  25 in total

1.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

2.  Cloning and sequencing of the PHO80 gene and CEN15 of Saccharomyces cerevisiae.

Authors:  A Toh-e; T Shimauchi
Journal:  Yeast       Date:  1986-06       Impact factor: 3.239

3.  A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Authors:  H C Birnboim; J Doly
Journal:  Nucleic Acids Res       Date:  1979-11-24       Impact factor: 16.971

4.  In vitro mutagenesis of a circular DNA molecule by using synthetic restriction sites.

Authors:  F Heffron; M So; B J McCarthy
Journal:  Proc Natl Acad Sci U S A       Date:  1978-12       Impact factor: 11.205

5.  Transformation in yeast: development of a hybrid cloning vector and isolation of the CAN1 gene.

Authors:  J R Broach; J N Strathern; J B Hicks
Journal:  Gene       Date:  1979-12       Impact factor: 3.688

6.  Isolation of yeast histone genes H2A and H2B.

Authors:  L Hereford; K Fahrner; J Woolford; M Rosbash; D B Kaback
Journal:  Cell       Date:  1979-12       Impact factor: 41.582

7.  A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae.

Authors:  A Toh-E; H Nakamura; Y Oshima
Journal:  Biochim Biophys Acta       Date:  1976-03-25

8.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  Characterization of a dominant, constitutive mutation, PHOO, for the repressible acid phosphatase synthesis in Saccharomyces cerevisiae.

Authors:  A Toh-E; Y Oshima
Journal:  J Bacteriol       Date:  1974-11       Impact factor: 3.490
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  30 in total

1.  Molecular and expression analysis of the negative regulators involved in the transcriptional regulation of acid phosphatase production in Saccharomyces cerevisiae.

Authors:  S L Madden; D L Johnson; L W Bergman
Journal:  Mol Cell Biol       Date:  1990-11       Impact factor: 4.272

2.  Cyclin partners determine Pho85 protein kinase substrate specificity in vitro and in vivo: control of glycogen biosynthesis by Pcl8 and Pcl10.

Authors:  D Huang; J Moffat; W A Wilson; L Moore; C Cheng; P J Roach; B Andrews
Journal:  Mol Cell Biol       Date:  1998-06       Impact factor: 4.272

3.  NUC-2, a component of the phosphate-regulated signal transduction pathway in Neurospora crassa, is an ankyrin repeat protein.

Authors:  Y Poleg; R Aramayo; S Kang; J G Hall; R L Metzenberg
Journal:  Mol Gen Genet       Date:  1996-10-28

4.  PHO85, a negative regulator of the PHO system, is a homolog of the protein kinase gene, CDC28, of Saccharomyces cerevisiae.

Authors:  A Toh-e; K Tanaka; Y Uesono; R B Wickner
Journal:  Mol Gen Genet       Date:  1988-09

Review 5.  Redundancy or specificity? The role of the CDK Pho85 in cell cycle control.

Authors:  Javier Jiménez; Natalia Ricco; Carmen Grijota-Martínez; Rut Fadó; Josep Clotet
Journal:  Int J Biochem Mol Biol       Date:  2013-09-13

6.  Phosphorylation of sic1, a cyclin-dependent kinase (Cdk) inhibitor, by Cdk including Pho85 kinase is required for its prompt degradation.

Authors:  M Nishizawa; M Kawasumi; M Fujino; A Toh-e
Journal:  Mol Biol Cell       Date:  1998-09       Impact factor: 4.138

7.  The PHOA and PHOB cyclin-dependent kinases perform an essential function in Aspergillus nidulans.

Authors:  Xiaowei Dou; Dongliang Wu; Weiling An; Jonathan Davies; Shahr B Hashmi; Leena Ukil; Stephen A Osmani
Journal:  Genetics       Date:  2003-11       Impact factor: 4.562

Review 8.  Regulation of Cdc28 cyclin-dependent protein kinase activity during the cell cycle of the yeast Saccharomyces cerevisiae.

Authors:  M D Mendenhall; A E Hodge
Journal:  Microbiol Mol Biol Rev       Date:  1998-12       Impact factor: 11.056

Review 9.  Controlling transcription by destruction: the regulation of yeast Gcn4p stability.

Authors:  Stefan Irniger; Gerhard H Braus
Journal:  Curr Genet       Date:  2003-07-09       Impact factor: 3.886

10.  Negative regulators of the PHO system of Saccharomyces cerevisiae: characterization of PHO80 and PHO85.

Authors:  Y Uesono; M Tokai; K Tanaka; A Tohe
Journal:  Mol Gen Genet       Date:  1992-02
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