Literature DB >> 3526348

Spermidine synthase of Escherichia coli: localization of the speE gene.

C W Tabor, H Tabor, Q W Xie.   

Abstract

We have obtained Escherichia coli mutants lacking spermidine synthase (putrescine aminopropyltransferase) and have found that the mutated gene (speE) is located immediately upstream from the gene coding for S-adenosylmethionine decarboxylase (speD); these genes are located at 2.7 minutes on the E. coli chromosome. Both genes are present in a 1795-base-pair fragment of E. coli DNA that was cloned into pBR322. Deletion of 105 bases upstream of speE caused a coordinate loss of both activities, indicating that speE and speD constitute a single operon. speE and speD have also been cloned separately in a high-expression vector; strains carrying these plasmids overproduce the respective enzymes.

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Year:  1986        PMID： 3526348      PMCID： PMC386433          DOI： 10.1073/pnas.83.16.6040

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors:  H J VOGEL; D M BONNER
Journal:  J Biol Chem       Date:  1956-01       Impact factor: 5.157

2.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

3.  Filamentous coliphage M13 as a cloning vehicle: insertion of a HindII fragment of the lac regulatory region in M13 replicative form in vitro.

Authors:  J Messing; B Gronenborn; B Müller-Hill; P Hans Hopschneider
Journal:  Proc Natl Acad Sci U S A       Date:  1977-09       Impact factor: 11.205

4.  Mapping of genes involved in the synthesis of spermidine in Escherichia coli.

Authors:  W K Maas
Journal:  Mol Gen Genet       Date:  1972

5.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

6.  Escherichia coli mutants completely deficient in adenosylmethionine decarboxylase and in spermidine biosynthesis.

Authors:  C W Tabor; H Tabor; E W Hafner
Journal:  J Biol Chem       Date:  1978-05-25       Impact factor: 5.157

7.  Mutants of Escherichia coli that do not contain 1,4-diaminobutane (putrescine) or spermidine.

Authors:  E W Hafner; C W Tabor; H Tabor
Journal:  J Biol Chem       Date:  1979-12-25       Impact factor: 5.157

8.  S-adenosylmethionine decarboxylase of Escherichia coli. Studies on the covalently linked pyruvate required for activity.

Authors:  G D Markham; C W Tabor; H Tabor
Journal:  J Biol Chem       Date:  1982-10-25       Impact factor: 5.157

9.  DNA sequencing with chain-terminating inhibitors.

Authors:  F Sanger; S Nicklen; A R Coulson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

10.  O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

Authors:  J R Benson; P E Hare
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205
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  21 in total

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Review 2.  Linkage map of Escherichia coli K-12, edition 10: the traditional map.

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Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

Review 3.  Linkage map of Escherichia coli K-12, edition 8.

Authors:  B J Bachmann
Journal:  Microbiol Rev       Date:  1990-06

4.  Identification of a novel aminopropyltransferase involved in the synthesis of branched-chain polyamines in hyperthermophiles.

Authors:  Kazuma Okada; Ryota Hidese; Wakao Fukuda; Masaru Niitsu; Koichi Takao; Yuhei Horai; Naoki Umezawa; Tsunehiko Higuchi; Tairo Oshima; Yuko Yoshikawa; Tadayuki Imanaka; Shinsuke Fujiwara
Journal:  J Bacteriol       Date:  2014-03-07       Impact factor: 3.490

5.  Functional characterization of the potRABCD operon for spermine and spermidine uptake and regulation in Staphylococcus aureus.

Authors:  Xiangyu Yao; Chung-Dar Lu
Journal:  Curr Microbiol       Date:  2014-03-09       Impact factor: 2.188

6.  Gene expression in tobacco low-nicotine mutants.

Authors:  N Hibi; S Higashiguchi; T Hashimoto; Y Yamada
Journal:  Plant Cell       Date:  1994-05       Impact factor: 11.277

7.  In vivo mechanism-based inactivation of S-adenosylmethionine decarboxylases from Escherichia coli, Salmonella typhimurium, and Saccharomyces cerevisiae.

Authors:  Y F Li; S Hess; L K Pannell; C White Tabor; H Tabor
Journal:  Proc Natl Acad Sci U S A       Date:  2001-08-28       Impact factor: 11.205

8.  Spermidine biosynthesis in Escherichia coli: promoter and termination regions of the speED operon.

Authors:  Q W Xie; C W Tabor; H Tabor
Journal:  J Bacteriol       Date:  1989-08       Impact factor: 3.490

9.  Structure and activity of mouse S-adenosylmethionine decarboxylase gene promoters and properties of the encoded proteins.

Authors:  K Nishimura; M Liisanantti; Y Muta; K Kashiwagi; A Shirahata; M Jänne; K Kankare; O A Jänne; K Igarashi
Journal:  Biochem J       Date:  1998-06-15       Impact factor: 3.857

10.  Site-directed mutations of the gatekeeping loop region affect the activity of Escherichia coli spermidine synthase.

Authors:  Mon-Juan Lee; Ya-Ting Yang; Vivian Lin; Haimei Huang
Journal:  Mol Biotechnol       Date:  2013-06       Impact factor: 2.695
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