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Literature DB >> 1054843

O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

Abstract

O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.

Entities: Chemical

Mesh：

Substances：

Year: 1975 PMID： 1054843 PMCID： PMC432365 DOI： 10.1073/pnas.72.2.619

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

10 in total

1. Removal of a Fluoram 1-positive impurity from hydrochloric acid.

Authors: C Schwabe; J C Catlin
Journal: Anal Biochem Date: 1974-09 Impact factor: 3.365

2. A new sensitive, rapid fluorescence technique for the determination of proteins in gel electrophoresis and in solution.

Authors: E Weidekamm; D F Wallach; R Flückiger
Journal: Anal Biochem Date: 1973-07 Impact factor: 3.365

3. Total fluorometric amino acid analysis using fluorescamine.

Authors: A M Felix; G Terkelsen
Journal: Arch Biochem Biophys Date: 1973-07 Impact factor: 4.013

4. Column chromatography of amino acids with fluorescence detection.

Authors: M Roth; A Hampaï
Journal: J Chromatogr Date: 1973-08-29

5. Single column analysis of amino acids in protein hydrolysates utilizing the fluorescamine reaction.

Authors: A G Georgiadis; J W Coffey
Journal: Anal Biochem Date: 1973-11 Impact factor: 3.365

6. Lysosomal peptidase measurement by sensitive fluorometric amino acid analysis.

Authors: S Taylor; A L Tappel
Journal: Anal Biochem Date: 1973-11 Impact factor: 3.365

7. Determination of hydroxyproline in fluorometric amino acid analysis with fluorescamine.

Authors: A M Felix; G Terkelsen
Journal: Anal Biochem Date: 1973-12 Impact factor: 3.365

8. Ion-exchange chromatography in lunar organic analysis.

Authors: P E Hare
Journal: Space Life Sci Date: 1972-10

9. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

Authors: S Udenfriend; S Stein; P Böhlen; W Dairman; W Leimgruber; M Weigele
Journal: Science Date: 1972-11-24 Impact factor: 47.728

10. Fluorescence reaction for amino acids.

Authors: M Roth
Journal: Anal Chem Date: 1971-06 Impact factor: 6.986

10 in total

92 in total

O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

1. Removal of a Fluoram 1-positive impurity from hydrochloric acid.

2. A new sensitive, rapid fluorescence technique for the determination of proteins in gel electrophoresis and in solution.

3. Total fluorometric amino acid analysis using fluorescamine.

4. Column chromatography of amino acids with fluorescence detection.

5. Single column analysis of amino acids in protein hydrolysates utilizing the fluorescamine reaction.

6. Lysosomal peptidase measurement by sensitive fluorometric amino acid analysis.

7. Determination of hydroxyproline in fluorometric amino acid analysis with fluorescamine.

8. Ion-exchange chromatography in lunar organic analysis.

9. Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.

10. Fluorescence reaction for amino acids.

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