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Literature DB >> 3526337

Suppression of human DNA alkylation-repair defects by Escherichia coli DNA-repair genes.

Abstract

The ada-alkB operon protects Escherichia coli against the effects of many alkylating agents. We have subcloned it into the pSV2 mammalian expression vector to yield pSV2ada-alkB, and this plasmid has been introduced into Mer- HeLa S3 cells, which are extremely sensitive to killing and induction of sister chromatid exchange by alkylating agents. One transformant (the S3-9 cell line) has several integrated copies of pSV2ada-alkB and was found to express a very high level of the ada gene product, the 39-kDa O6-methylguanine-DNA methyltransferase. S3-9 cells were found to have become resistant to killing and induction of sister chromatid exchange by two alkylating agents, N-methyl-N'-nitro-N-nitrosoguanidine and N,N'-bis(2-chloroethyl)-N-nitro-sourea. This shows that bacterial DNA alkylation-repair genes are able to suppress the alkylation-repair defects in human Mer- cells.

Entities: Chemical Gene Species

Mesh：

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Year: 1986 PMID： 3526337 PMCID： PMC386337 DOI： 10.1073/pnas.83.15.5607

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

29 in total

1. Methyl phosphotriesters in alkylated DNA are repaired by the Ada regulatory protein of E. coli.

Authors: T V McCarthy; T Lindahl
Journal: Nucleic Acids Res Date: 1985-04-25 Impact factor: 16.971

2. Induction and autoregulation of ada, a positively acting element regulating the response of Escherichia coli K-12 to methylating agents.

Authors: P K Lemotte; G C Walker
Journal: J Bacteriol Date: 1985-03 Impact factor: 3.490

3. Genetic complementation of UV-induced DNA repair in Chinese hamster ovary cells by the denV gene of phage T4.

Authors: K Valerie; J K de Riel; E E Henderson
Journal: Proc Natl Acad Sci U S A Date: 1985-11 Impact factor: 11.205

4. In vivo mutagenesis by O6-methylguanine built into a unique site in a viral genome.

Authors: E L Loechler; C L Green; J M Essigmann
Journal: Proc Natl Acad Sci U S A Date: 1984-10 Impact factor: 11.205

5. The cytotoxic and mutagenic effects of alkylating agents on human lymphoid cells are caused by different DNA lesions.

Authors: P Karran; S A Williams
Journal: Carcinogenesis Date: 1985-05 Impact factor: 4.944

6. Expression of the E. coli O6-methylguanine-methylphosphotriester methyltransferase gene in mammalian cells.

Authors: J Brennand; G P Margison
Journal: Carcinogenesis Date: 1986-01 Impact factor: 4.944

7. DNA-mediated transfer and expression of a human DNA repair gene that demethylates O6-methylguanine.

Authors: R Ding; K Ghosh; A Eastman; E Bresnick
Journal: Mol Cell Biol Date: 1985-11 Impact factor: 4.272

8. Depletion of O6-methylguanine-DNA-methyltransferase in human fibroblasts increases the mutagenic response to N-methyl-N'-nitro-N-nitrosoguanidine.

Authors: J Domoradzki; A E Pegg; M E Dolan; V M Maher; J J McCormick
Journal: Carcinogenesis Date: 1985-12 Impact factor: 4.944

9. Possible depletion of a DNA repair enzyme in human lymphoma cells by subversive repair.

Authors: P Karran
Journal: Proc Natl Acad Sci U S A Date: 1985-08 Impact factor: 11.205

10. Cloning of the E. coli O6-methylguanine and methylphosphotriester methyltransferase gene using a functional DNA repair assay.

Authors: G P Margison; D P Cooper; J Brennand
Journal: Nucleic Acids Res Date: 1985-03-25 Impact factor: 16.971

33 in total

Review 1. How heterologously expressed Escherichia coli genes contribute to understanding DNA repair processes in Saccharomyces cerevisiae.

Authors: Jela Brozmanová; Viera Vlcková; Miroslav Chovanec
Journal: Curr Genet Date: 2004-11-13 Impact factor: 3.886

2. Transfer of human genes conferring resistance to methylating mutagens, but not to UV irradiation and cross-linking agents, into Chinese hamster ovary cells.

Authors: B Kaina; A A Van Zeeland; C Backendorf; H W Thielmann; P Van de Putte
Journal: Mol Cell Biol Date: 1987-05 Impact factor: 4.272

3. DNA polymerase II (polB) is involved in a new DNA repair pathway for DNA interstrand cross-links in Escherichia coli.

Authors: M Berardini; P L Foster; E L Loechler
Journal: J Bacteriol Date: 1999-05 Impact factor: 3.490

4. Evidence from in vitro replication that O6-methylguanine can adopt multiple conformations.

Authors: M K Dosanjh; E L Loechler; B Singer
Journal: Proc Natl Acad Sci U S A Date: 1993-05-01 Impact factor: 11.205

5. Characterization of the major DNA repair methyltransferase activity in unadapted Escherichia coli and identification of a similar activity in Salmonella typhimurium.

Authors: G W Rebeck; C M Smith; D L Goad; L Samson
Journal: J Bacteriol Date: 1989-09 Impact factor: 3.490

6. Pediatric brain tumors: mutations of two dioxygenases (hABH2 and hABH3) that directly repair alkylation damage.

Authors: Valentina Cetica; Lorenzo Genitori; Laura Giunti; Massimiliano Sanzo; Gabriella Bernini; Maura Massimino; Iacopo Sardi
Journal: J Neurooncol Date: 2009-03-17 Impact factor: 4.130

7. Cisplatin-DNA adducts are molecular decoys for the ribosomal RNA transcription factor hUBF (human upstream binding factor).

Authors: D K Treiber; X Zhai; H M Jantzen; J M Essigmann
Journal: Proc Natl Acad Sci U S A Date: 1994-06-07 Impact factor: 11.205