| Literature DB >> 35253881 |
Weijie Zhao1, Xiaoyan Wang1, Qian Zhang1, Qian Zheng1, Haitao Yao1, Xiangyang Gu1, Dongliang Liu1, Xuemin Tian1, Xiaoji Wang1, Yongqing Li2,3, Zhengge Zhu1.
Abstract
The homeostasis of histone methylation is maintained by histone methyltransferases and demethylases, which are important for the regulation of gene expression. Here, we report a histone demethylase from rice (Oryza sativa), Jumonji C domain-containing protein (JMJ710), which belongs to the JMJD6 group and plays an important role in the response to drought stress. Overexpression of JMJ710 causes a drought-sensitive phenotype, while RNAi and clustered regularly interspaced short palindromic repeats (CRISPR)-knockout mutant lines show drought tolerance. In vitro and in vivo assays showed that JMJ710 is a histone demethylase. It targets to MYB TRANSCRIPTION FACTOR 48 (MYB48-1) chromatin, demethylates H3K36me2, and negatively regulates the expression of MYB48-1, a positive regulator of drought tolerance. Under drought stress, JMJ710 is downregulated and the expression of MYB48-1 increases, and the subsequent activation of its downstream drought-responsive genes leads to drought tolerance. This research reports a negative regulator of drought stress-responsive genes, JMJ710, that ensures that the drought tolerance mechanism is not mis-activated under normal conditions but allows quick activation upon drought stress. © American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.Entities:
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Year: 2022 PMID： 35253881 PMCID： PMC9157158 DOI： 10.1093/plphys/kiac095
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.005