Literature DB >> 35218546

Measuring the Lateral Diffusion of Plasma Membrane Receptors Using Raster Image Correlation Spectroscopy.

Sara Makaremi1, Jose Moran-Mirabal2,3.   

Abstract

Raster image correlation spectroscopy (RICS) enables detecting and quantifying diffusion in live cells using standard commercial laser scanning confocal microscopes. Here, we describe a protocol based on RICS for measuring the lateral diffusion of two immunoreceptors within the plasma membrane of the macrophage cell line RAW 264.7. The sample images and measurements presented in this chapter were obtained from RICS analysis of Toll-like receptor 2 (TLR2) and cluster of differentiation 14 (CD14), which are transmembrane and membrane-anchored receptors, respectively. A step-by-step guideline is provided to acquire raster-scanned images and to extract the diffusion coefficients using RICS analysis.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  CD14; Immune receptor; Macrophage; RAW 264.7; Receptor mobility; TLR2

Mesh：

Year:  2022        PMID： 35218546     DOI： 10.1007/978-1-0716-2051-9_17

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  13 in total

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Authors:  Carlo Manzo; Maria F Garcia-Parajo
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2.  Measuring fast dynamics in solutions and cells with a laser scanning microscope.

Authors:  Michelle A Digman; Claire M Brown; Parijat Sengupta; Paul W Wiseman; Alan R Horwitz; Enrico Gratton
Journal:  Biophys J       Date:  2005-05-20       Impact factor: 4.033

3.  Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure.

Authors:  Michelle A Digman; Parijat Sengupta; Paul W Wiseman; Claire M Brown; Alan R Horwitz; Enrico Gratton
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Review 4.  Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells.

Authors:  David L Kolin; Paul W Wiseman
Journal:  Cell Biochem Biophys       Date:  2007-10-02       Impact factor: 2.194

5.  Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope.

Authors:  C M Brown; R B Dalal; B Hebert; M A Digman; A R Horwitz; E Gratton
Journal:  J Microsc       Date:  2008-01       Impact factor: 1.758

6.  Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method.

Authors:  Michelle A Digman; Paul W Wiseman; Alan R Horwitz; Enrico Gratton
Journal:  Biophys J       Date:  2009-01       Impact factor: 4.033

7.  Fluorescence correlation spectroscopy. II. An experimental realization.

Authors:  D Magde; E L Elson; W W Webb
Journal:  Biopolymers       Date:  1974-01       Impact factor: 2.505

8.  Theoretical analysis of fluorescence photobleaching recovery experiments.

Authors:  D M Soumpasis
Journal:  Biophys J       Date:  1983-01       Impact factor: 4.033

9.  Analysis of diffusion and binding in cells using the RICS approach.

Authors:  Michelle A Digman; Enrico Gratton
Journal:  Microsc Res Tech       Date:  2009-04       Impact factor: 2.769

10.  Lateral diffusion of CD14 and TLR2 in macrophage plasma membrane assessed by raster image correlation spectroscopy and single particle tracking.

Authors:  Sara Makaremi; Markus Rose; Suman Ranjit; Michelle A Digman; Dawn M E Bowdish; Jose M Moran-Mirabal
Journal:  Sci Rep       Date:  2020-11-09       Impact factor: 4.379
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