Literature DB >> 35218542

Expansion Microscopy of Larval Zebrafish Brains and Zebrafish Embryos.

Ory Perelsman1, Shoh Asano2, Limor Freifeld3.   

Abstract

Since its introduction in 2015, expansion microscopy (ExM) allowed imaging a broad variety of biological structures in many models, at nanoscale resolution. Here, we describe in detail a protocol for application of ExM in whole-brains of zebrafish larvae and intact embryos, and discuss the considerations involved in the imaging of nonflat, whole-organ or organism samples, more broadly.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Development; Expansion microscopy; Neuroscience; Superresolution microscopy; Zebrafish

Mesh：

Year:  2022        PMID： 35218542     DOI： 10.1007/978-1-0716-2051-9_13

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  19 in total

1.  Glia Accumulate Evidence that Actions Are Futile and Suppress Unsuccessful Behavior.

Authors:  Yu Mu; Davis V Bennett; Mikail Rubinov; Sujatha Narayan; Chao-Tsung Yang; Masashi Tanimoto; Brett D Mensh; Loren L Looger; Misha B Ahrens
Journal:  Cell       Date:  2019-06-20       Impact factor: 41.582

Review 2.  Expansion Microscopy: Scalable and Convenient Super-Resolution Microscopy.

Authors:  Paul W Tillberg; Fei Chen
Journal:  Annu Rev Cell Dev Biol       Date:  2019-08-19       Impact factor: 13.827

3.  Optical imaging. Expansion microscopy.

Authors:  Fei Chen; Paul W Tillberg; Edward S Boyden
Journal:  Science       Date:  2015-01-15       Impact factor: 47.728

4.  Expansion Microscopy: Protocols for Imaging Proteins and RNA in Cells and Tissues.

Authors:  Shoh M Asano; Ruixuan Gao; Asmamaw T Wassie; Paul W Tillberg; Fei Chen; Edward S Boyden
Journal:  Curr Protoc Cell Biol       Date:  2018-08-02

5.  Expansion microscopy with conventional antibodies and fluorescent proteins.

Authors:  Tyler J Chozinski; Aaron R Halpern; Haruhisa Okawa; Hyeon-Jin Kim; Grant J Tremel; Rachel O L Wong; Joshua C Vaughan
Journal:  Nat Methods       Date:  2016-04-11       Impact factor: 28.547

6.  Expansion microscopy of zebrafish for neuroscience and developmental biology studies.

Authors:  Limor Freifeld; Iris Odstrcil; Dominique Förster; Alyson Ramirez; James A Gagnon; Owen Randlett; Emma K Costa; Shoh Asano; Orhan T Celiker; Ruixuan Gao; Daniel A Martin-Alarcon; Paul Reginato; Cortni Dick; Linlin Chen; David Schoppik; Florian Engert; Herwig Baier; Edward S Boyden
Journal:  Proc Natl Acad Sci U S A       Date:  2017-11-21       Impact factor: 11.205

7.  Nuclear microenvironments modulate transcription from low-affinity enhancers.

Authors:  Albert Tsai; Justin Crocker; Anand K Muthusamy; Mariana Rp Alves; Luke D Lavis; Robert H Singer; David L Stern
Journal:  Elife       Date:  2017-11-02       Impact factor: 8.140

8.  Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy.

Authors:  Yongxin Zhao; Octavian Bucur; Humayun Irshad; Fei Chen; Astrid Weins; Andreea L Stancu; Eun-Young Oh; Marcello DiStasio; Vanda Torous; Benjamin Glass; Isaac E Stillman; Stuart J Schnitt; Andrew H Beck; Edward S Boyden
Journal:  Nat Biotechnol       Date:  2017-07-17       Impact factor: 54.908

9.  Protein-retention expansion microscopy of cells and tissues labeled using standard fluorescent proteins and antibodies.

Authors:  Paul W Tillberg; Fei Chen; Kiryl D Piatkevich; Yongxin Zhao; Chih-Chieh Jay Yu; Brian P English; Linyi Gao; Anthony Martorell; Ho-Jun Suk; Fumiaki Yoshida; Ellen M DeGennaro; Douglas H Roossien; Guanyu Gong; Uthpala Seneviratne; Steven R Tannenbaum; Robert Desimone; Dawen Cai; Edward S Boyden
Journal:  Nat Biotechnol       Date:  2016-07-04       Impact factor: 54.908

10.  Whole-brain activity mapping onto a zebrafish brain atlas.

Authors:  Owen Randlett; Caroline L Wee; Eva A Naumann; Onyeka Nnaemeka; David Schoppik; James E Fitzgerald; Ruben Portugues; Alix M B Lacoste; Clemens Riegler; Florian Engert; Alexander F Schier
Journal:  Nat Methods       Date:  2015-11       Impact factor: 28.547
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.