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Literature DB >> 3519546

An immunofluorescent method for a specific demonstration of granulocytes and some of their proteins (ECP and CCP).

Abstract

Using p-benzoquinone as a fixative the non-specific fluorescence of granulocytes and especially the eosinophils is removed for both FITC and TRITC. In this way we have been able to detect the eosinophil cationic protein (ECP) and the chymotrypsin-like cationic protein (CCP) in human lung tissue and by double immunofluorescent labelling shown that these two proteins very likely are related to eosinophils respectively the neutrophils.

Entities: Chemical Gene Species

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Year: 1986 PMID： 3519546 DOI： 10.1007/bf00495790

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

11 in total

1. Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.

Authors: A G Pearse; J M Polak
Journal: Histochem J Date: 1975-03

2. Abolition of non-specific fluorescent staining of eosinophils.

Authors: N W Johnston; J Bienenstock
Journal: J Immunol Methods Date: 1974-03 Impact factor: 2.303

3. The reaction of benzoquinone with amines and proteins.

Authors: M Morrison; W Steele; D J Danner
Journal: Arch Biochem Biophys Date: 1969-11 Impact factor: 4.013

4. An immunofluorescent method for specific staining of eosinophil granule major basic protein.

Authors: W V Filley; S J Ackerman; G J Gleich
Journal: J Immunol Methods Date: 1981 Impact factor: 2.303

5. Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukemic myeloid cells.

Authors: I Olsson; P Venge
Journal: Blood Date: 1974-08 Impact factor: 22.113

6. Ultrastructural localization of a new neuronal peptide (VIP).

Authors: L I Larsson
Journal: Histochemistry Date: 1977-10-22

7. Identification by immunofluorescence of eosinophil granule major basic protein in lung tissues of patients with bronchial asthma.

Authors: W V Filley; K E Holley; G M Kephart; G J Gleich
Journal: Lancet Date: 1982-07-03 Impact factor: 79.321

An immunofluorescent method for a specific demonstration of granulocytes and some of their proteins (ECP and CCP).

1. Bifunctional reagents as vapour- and liquid-phase fixatives for immunohistochemistry.

2. Abolition of non-specific fluorescent staining of eosinophils.

3. The reaction of benzoquinone with amines and proteins.

4. An immunofluorescent method for specific staining of eosinophil granule major basic protein.

5. Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukemic myeloid cells.

6. Ultrastructural localization of a new neuronal peptide (VIP).

7. Identification by immunofluorescence of eosinophil granule major basic protein in lung tissues of patients with bronchial asthma.

8. Radioimmunoassay of human eosinophil cationic protein.

9. Immunofluorescence identification of eosinophil granule major basic protein in the flame figures of Wells' syndrome.

10. Localization of eosinophil granule major basic protein in chronic urticaria.

1. A novel fixative for immunofluorescence staining of CD133-positive glioblastoma stem cells.

2. TRIM11 is overexpressed in high-grade gliomas and promotes proliferation, invasion, migration and glial tumor growth.