| Literature DB >> 21402102 |
Jonathan H Sherman1, Gerard T Redpath, Jan A Redick, Benjamin W Purow, Edward R Laws, John A Jane, Mark E Shaffrey, Isa M Hussaini.
Abstract
Isolation of glioblastoma stem cells requires incubation of tumor cells in a neural stem cell media. Neurospheres containing these glioblastoma stem cells are formed after approximately a five-day period. These cells can then be analyzed for the presence of stem cell markers. Immunofluorescence staining for these markers can serve as a valuable tool for analyzing the intact neurosphere directly in stem cell media. Here we present the use of a novel fixative (1,4-benzoquinone) for immunoflourescence staining of neurospheres.Entities:
Mesh：
Substances：
Year: 2011 PMID： 21402102 PMCID： PMC3126626 DOI： 10.1016/j.jneumeth.2011.03.003
Source DB: PubMed Journal: J Neurosci Methods ISSN： 0165-0270 Impact factor: 2.390