Literature DB >> 35171491

Image-Based Screening for Stress Granule Regulators.

Katharina Hoerth1,2, Nina Eiermann1,2, Jürgen Beneke3,4, Holger Erfle3,4, Georg Stoecklin5,6,7.   

Abstract

Stress granule (SG)-based RNA interference (RNAi) screening is a powerful method to discover factors that control protein synthesis and aggregation, as well as regulators of SG assembly and disassembly. Here, we describe how to set up and optimize a large-scale siRNA screen, and give a detailed outline for the automated quantification of SGs as a visual readout. Hit evaluation via calculated Z scores provides a list of candidates for further in-depth studies.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Automated image analysis; Microscopic screen; Stress granule; Z score; siRNA

Mesh：

Substances：

Year:  2022        PMID： 35171491     DOI： 10.1007/978-1-0716-1975-9_22

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  31 in total

Review 1.  Stress granules and cell signaling: more than just a passing phase?

Authors:  Nancy Kedersha; Pavel Ivanov; Paul Anderson
Journal:  Trends Biochem Sci       Date:  2013-09-10       Impact factor: 13.807

2.  Coordination of translational control and protein homeostasis during severe heat stress.

Authors:  Valeria Cherkasov; Sarah Hofmann; Silke Druffel-Augustin; Axel Mogk; Jens Tyedmers; Georg Stoecklin; Bernd Bukau
Journal:  Curr Biol       Date:  2013-11-27       Impact factor: 10.834

3.  A high-content screen identifies novel compounds that inhibit stress-induced TDP-43 cellular aggregation and associated cytotoxicity.

Authors:  Justin D Boyd; Peter Lee; Marisa S Feiler; Nava Zauur; Min Liu; John Concannon; Atsushi Ebata; Benjamin Wolozin; Marcie A Glicksman
Journal:  J Biomol Screen       Date:  2013-09-09

Review 4.  Cytoplasmic stress granules: Dynamic modulators of cell signaling and disease.

Authors:  Hicham Mahboubi; Ursula Stochaj
Journal:  Biochim Biophys Acta Mol Basis Dis       Date:  2017-01-15       Impact factor: 5.187

5.  G3BP1 Is a Tunable Switch that Triggers Phase Separation to Assemble Stress Granules.

Authors:  Peiguo Yang; Cécile Mathieu; Regina-Maria Kolaitis; Peipei Zhang; James Messing; Ugur Yurtsever; Zemin Yang; Jinjun Wu; Yuxin Li; Qingfei Pan; Jiyang Yu; Erik W Martin; Tanja Mittag; Hong Joo Kim; J Paul Taylor
Journal:  Cell       Date:  2020-04-16       Impact factor: 41.582

6.  Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD.

Authors:  Mark Y Fang; Sebastian Markmiller; Anthony Q Vu; Ashkan Javaherian; William E Dowdle; Philippe Jolivet; Paul J Bushway; Nicholas A Castello; Ashmita Baral; Michelle Y Chan; Jeremy W Linsley; Drew Linsley; Mark Mercola; Steven Finkbeiner; Eric Lecuyer; Joseph W Lewcock; Gene W Yeo
Journal:  Neuron       Date:  2019-07-01       Impact factor: 17.173

7.  A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly.

Authors:  Takbum Ohn; Nancy Kedersha; Tyler Hickman; Sarah Tisdale; Paul Anderson
Journal:  Nat Cell Biol       Date:  2008-09-14       Impact factor: 28.824

Review 8.  Differences between acute and chronic stress granules, and how these differences may impact function in human disease.

Authors:  Lucas C Reineke; Joel R Neilson
Journal:  Biochem Pharmacol       Date:  2018-10-14       Impact factor: 5.858

9.  Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors.

Authors:  Emily C Wheeler; Anthony Q Vu; Jaclyn M Einstein; Matthew DiSalvo; Noorsher Ahmed; Eric L Van Nostrand; Alexander A Shishkin; Wenhao Jin; Nancy L Allbritton; Gene W Yeo
Journal:  Nat Methods       Date:  2020-05-11       Impact factor: 28.547

10.  CDK1 couples proliferation with protein synthesis.

Authors:  Katharina Haneke; Johanna Schott; Doris Lindner; Anne Kruse Hollensen; Christian Kroun Damgaard; Cyril Mongis; Michael Knop; Wilhelm Palm; Alessia Ruggieri; Georg Stoecklin
Journal:  J Cell Biol       Date:  2020-03-02       Impact factor: 10.539
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