Literature DB >> 35171490

Single-Molecule Imaging of mRNA Interactions with Stress Granules.

Tatsuya Morisaki1, Timothy J Stasevich2,3.   

Abstract

Single-molecule imaging in living cells enables the investigation of molecular dynamics and interactions underlying the physiology of a cell. We recently developed a method to visualize translation events at single-mRNA resolution in living cells. Here we describe how we apply this method to visualize mRNA interactions with stress granules in the context of translational activity during cell stress.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  MS2; RNA imaging; Single-molecule imaging; Stress granules; Translation

Mesh：

Substances：

Year:  2022        PMID： 35171490      PMCID： PMC9191879          DOI： 10.1007/978-1-0716-1975-9_21

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  26 in total

1.  Real-time observation of transcription initiation and elongation on an endogenous yeast gene.

Authors:  Daniel R Larson; Daniel Zenklusen; Bin Wu; Jeffrey A Chao; Robert H Singer
Journal:  Science       Date:  2011-04-22       Impact factor: 47.728

2.  LambdaN-GFP: an RNA reporter system for live-cell imaging.

Authors:  Nathalie Daigle; Jan Ellenberg
Journal:  Nat Methods       Date:  2007-07-01       Impact factor: 28.547

3.  Highly inclined thin illumination enables clear single-molecule imaging in cells.

Authors:  Makio Tokunaga; Naoko Imamoto; Kumiko Sakata-Sogawa
Journal:  Nat Methods       Date:  2008-01-06       Impact factor: 28.547

4.  TrackMate: An open and extensible platform for single-particle tracking.

Authors:  Jean-Yves Tinevez; Nick Perry; Johannes Schindelin; Genevieve M Hoopes; Gregory D Reynolds; Emmanuel Laplantine; Sebastian Y Bednarek; Spencer L Shorte; Kevin W Eliceiri
Journal:  Methods       Date:  2016-10-03       Impact factor: 3.608

5.  Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals.

Authors:  James M Halstead; Timothée Lionnet; Johannes H Wilbertz; Frank Wippich; Anne Ephrussi; Robert H Singer; Jeffrey A Chao
Journal:  Science       Date:  2015-03-20       Impact factor: 47.728

Review 6.  Eukaryotic stress granules: the ins and outs of translation.

Authors:  J Ross Buchan; Roy Parker
Journal:  Mol Cell       Date:  2009-12-25       Impact factor: 17.970

7.  A general method to improve fluorophores for live-cell and single-molecule microscopy.

Authors:  Jonathan B Grimm; Brian P English; Jiji Chen; Joel P Slaughter; Zhengjian Zhang; Andrey Revyakin; Ronak Patel; John J Macklin; Davide Normanno; Robert H Singer; Timothée Lionnet; Luke D Lavis
Journal:  Nat Methods       Date:  2015-01-19       Impact factor: 28.547

8.  Dynamics of Translation of Single mRNA Molecules In Vivo.

Authors:  Xiaowei Yan; Tim A Hoek; Ronald D Vale; Marvin E Tanenbaum
Journal:  Cell       Date:  2016-05-05       Impact factor: 41.582

9.  Visualization of single endogenous polysomes reveals the dynamics of translation in live human cells.

Authors:  Xavier Pichon; Amandine Bastide; Adham Safieddine; Racha Chouaib; Aubin Samacoits; Eugenia Basyuk; Marion Peter; Florian Mueller; Edouard Bertrand
Journal:  J Cell Biol       Date:  2016-09-05       Impact factor: 10.539

10.  An improved MS2 system for accurate reporting of the mRNA life cycle.

Authors:  Evelina Tutucci; Maria Vera; Jeetayu Biswas; Jennifer Garcia; Roy Parker; Robert H Singer
Journal:  Nat Methods       Date:  2017-11-13       Impact factor: 28.547
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