| Literature DB >> 17603490 |
Nathalie Daigle1, Jan Ellenberg.
Abstract
We describe a GFP-based RNA reporter system (lambdaN-GFP) to visualize RNA molecules in live mammalian cells. It consists of GFP fused to an arginine-rich peptide derived from the phage lambda N protein, lambdaN22, which binds a unique minimal RNA motif and can be used to tag any RNA molecule. LambdaN-GFP uses a small and easy to engineer RNA tag, reducing the likelihood of perturbing the function of the tagged RNA molecule.Entities:
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Year: 2007 PMID： 17603490 DOI： 10.1038/nmeth1065
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547