| Literature DB >> 3512723 |
A Johannsson, D H Ellis, D L Bates, A M Plumb, C J Stanley.
Abstract
A method is described for increasing the response of enzyme immunoassays employing alkaline phosphatase as the label initiating 2 sequential catalytic reactions. First, NADP is dephosphorylated to produce NAD, which catalytically activates a specific redox-cycle involving the enzymes alcohol dehydrogenase and diaphorase. During each turn of the cycle 1 molecule of a tetrazolium salt is reduced to an intensely coloured formazan. The method is capable of detecting as little as 0.01 amol alkaline phosphatase, and when applied to an immunoassay for TSH a sensitivity (zero + 2.5 standard deviations) of 0.0013 mIU/l was obtained.Entities:
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Year: 1986 PMID： 3512723 DOI： 10.1016/0022-1759(86)90337-6
Source DB: PubMed Journal: J Immunol Methods ISSN： 0022-1759 Impact factor: 2.303