Literature DB >> 2048904

Amplification immunoassay for the determination of hepatitis B surface antigen.

A Seki1, E Tamiya, I Karube.   

Abstract

A sensitive sandwich immunoassay for the determination of Hepatitis B surface antigen (HBs) was developed, using a cascade system of Limulus amebocyte lysate as a signal amplification system. Lipopolysaccharide (LPS) was conjugated to anti-HBs antibody. Anti-HBs antibody was adsorbed to polystyrene beads. First, HBs were reacted to solid phase anti-HBs antibody (a-HBs). After the reaction, the beads were rinsed, and were then reacted with a-HBs-LPS. Then, LPS activity specifically bound to the beads was measured. HBs could be measured in the range of 10(-10)-10(-12) g/mL.

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Year:  1991        PMID： 2048904     DOI： 10.1007/bf02921540

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  12 in total

1.  An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label.

Authors:  S Comoglio; F Celada
Journal:  J Immunol Methods       Date:  1976-03       Impact factor: 2.303

2.  Determinations of proteins and hormones in serum by an immunoassay using antigen-enzyme conjugates.

Authors:  K Miedema; J Boelhouwer; J W Otten
Journal:  Clin Chim Acta       Date:  1972-08       Impact factor: 3.786

3.  Enzyme amplification for immunoassays. Detection limit of one hundredth of an attomole.

Authors:  A Johannsson; D H Ellis; D L Bates; A M Plumb; C J Stanley
Journal:  J Immunol Methods       Date:  1986-02-27       Impact factor: 2.303

4.  A new chromogenic endotoxin-specific assay using recombined limulus coagulation enzymes and its clinical applications.

Authors:  T Obayashi; H Tamura; S Tanaka; M Ohki; S Takahashi; M Arai; M Masuda; T Kawai
Journal:  Clin Chim Acta       Date:  1985-06-30       Impact factor: 3.786

5.  Extending the detection limit of solid-phase electrochemical enzyme immunoassay to the attomole level.

Authors:  S H Jenkins; W R Heineman; H B Halsall
Journal:  Anal Biochem       Date:  1988-02-01       Impact factor: 3.365

6.  Drug sensor: liposome immunosensor for theophylline.

Authors:  M Haga; S Sugawara; H Itagaki
Journal:  Anal Biochem       Date:  1981-12       Impact factor: 3.365

7.  Enzyme-linked immunoassay: conjugation of the Fab' fragment of rabbit IgG with beta-D-galactosidase from E. coli and its use for immunoassay.

Authors:  K Kato; H Fukui; Y Hamaguchi; E Ishikawa
Journal:  J Immunol       Date:  1976-06       Impact factor: 5.422

8.  Enzyme-labelled immunoassay for plasma cortisol.

Authors:  T Ogihara; K Miyal; K Nishi; K Ishibashi; Y Kumahara
Journal:  J Clin Endocrinol Metab       Date:  1977-01       Impact factor: 5.958

9.  Development of a sensitive enzyme immunoassay for human epidermal growth factor (urogastrone).

Authors:  M Kurobe; N Tokida; S Furukawa; E Ishikawa; K Hayashi
Journal:  Clin Chim Acta       Date:  1986-04-15       Impact factor: 3.786

10.  A fast highly sensitive colorimetric enzyme immunoassay system demonstrating benefits of enzyme amplification in clinical chemistry.

Authors:  A Johannsson; C J Stanley; C H Self
Journal:  Clin Chim Acta       Date:  1985-05-30       Impact factor: 3.786
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