Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin.

Literature DB >> 384908

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin.

Abstract

The enzyme-linked immunosorbent assay using the "double-sandwich" technique was utilized to determine Clostridium botulinum type E toxin. With this technique, about 80 mouse intraperitoneal 50% lethal doses of toxin could be detected. Cross-reaction was hardly observed with C. botulinum type A and B toxins. No cross-reaction was observed with culture supernatants of C. botulinum type C or other Clostridium strains. In all probability this was due to the high specificity of the antiserum prepared aginst the toxic component of type E toxin.

Entities: Disease Species

Mesh：

Substances：
Botulinum Toxins

Year: 1979 PMID： 384908 PMCID： PMC243373 DOI： 10.1128/aem.37.6.1173-1175.1979

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

8 in total

1. Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A.

Authors: S Notermans; J Dufrenne; M Schothorst
Journal: Jpn J Med Sci Biol Date: 1978-02

2. Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays.

Authors: E J Ruitenberg; P A Steerenberg; B J Brosi; J Buys
Journal: Bull World Health Organ Date: 1974 Impact factor: 9.408

3. Peroxidase-labeled antibody. A new method of conjugation.

Authors: P K Nakane; A Kawaoi
Journal: J Histochem Cytochem Date: 1974-12 Impact factor: 2.479

4. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

Authors: E Engvall; P Perlmann
Journal: J Immunol Date: 1972-07 Impact factor: 5.422

5. Purification and some properties of Clostridium botulinum type-E toxin.

Authors: M Kitamura; S Sakaguchi; G Sakaguchi
Journal: Biochim Biophys Acta Date: 1968-10-21

6. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

Authors: E Engvall; P Perlmann
Journal: Immunochemistry Date: 1971-09

7. Rapid bioassay for Clostridium botulinum type-E toxins by intravenous injection into mice.

Authors: G Sakaguchi; S Sakaguchi; H Kondo
Journal: Jpn J Med Sci Biol Date: 1968-12

8. The isolation of IgG from mammalian sera with the aid of caprylic acid.

Authors: M Steinbuch; R Audran
Journal: Arch Biochem Biophys Date: 1969-11 Impact factor: 4.013

8 in total

9 in total

Review 1. Clostridium botulinum neurotoxin.

Authors: H Sugiyama
Journal: Microbiol Rev Date: 1980-09

2. Detection of Clostridium botulinum type A toxin by enzyme-linked immunosorbent assay with antibodies produced in immunologically tolerant animals.

Authors: M Dezfulian; J G Bartlett
Journal: J Clin Microbiol Date: 1984-05 Impact factor: 5.948

Enzyme-linked immunosorbent assay for detection of Clostridium botulinum type E toxin.

1. Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A.

2. Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays.

3. Peroxidase-labeled antibody. A new method of conjugation.

4. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.

5. Purification and some properties of Clostridium botulinum type-E toxin.

6. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G.

7. Rapid bioassay for Clostridium botulinum type-E toxins by intravenous injection into mice.

8. The isolation of IgG from mammalian sera with the aid of caprylic acid.

Review 1. Clostridium botulinum neurotoxin.

2. Detection of Clostridium botulinum type A toxin by enzyme-linked immunosorbent assay with antibodies produced in immunologically tolerant animals.

3. Monoclonal antibody-based immunoassay for type A Clostridium botulinum toxin is comparable to the mouse bioassay.

4. Detection of Clostridium botulinum type G toxin by enzyme-linked immunosorbent assay.

5. Sensing the deadliest toxin: technologies for botulinum neurotoxin detection.

6. A simple, rapid and sensitive FRET assay for botulinum neurotoxin serotype B detection.

Review 7. Proteomic Methods of Detection and Quantification of Protein Toxins.

8. Commentary: A Cell Line for Detection of Botulinum Neurotoxin Type B.

9. Development and Characterization of Monoclonal Antibodies to Botulinum Neurotoxin Type E.