| Literature DB >> 35089561 |
Hoang D Nguyen1,2, Trang T P Phan3,4.
Abstract
Bacillus subtilis is a generally regarded as safe (GRAS) microorganism, which has been used for industrial production of recombinant enzymes. Many inducible and inducer-free expression vectors have been developed for intracellular production; some of those demonstrated the capability for protein expression up to 42% of total cellular proteins. In this chapter, we introduce the method to enhance the expression of soluble protein in B. subtilis. It includes the construction of vectors, the transformation of a plasmid into B. subtilis, and checking the expression of the protein.Entities:
Keywords: Bacillus subtilis expression vector; Pgrac promoter; Pgrac100 promoter; pHT01 vector; pHT255 vector
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Year: 2022 PMID： 35089561 DOI： 10.1007/978-1-0716-1859-2_14
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745