| Literature DB >> 16005967 |
Hoang Duc Nguyen1, Quynh Anh Nguyen, Rita C Ferreira, Luis C S Ferreira, Linh Thuoc Tran, Wolfgang Schumann.
Abstract
A series of plasmid-based expression vectors have been constructed allowing stable intracellular expression of recombinant proteins in Bacillus subtilis strains. These expression vectors are based on the recently described Escherichia coli-B. subtilis shuttle vector pMTLBS72 which replicates as theta circles. Besides the weak constitutive promoter P(lepA), we inserted three different controllable promoters: P(gsiB) which can be induced by heat and acid shock, and by ethanol, P(xylA) and P(spac) which respond to the addition of xylose and IPTG, respectively. The versatility of these expression vectors was demonstrated by fusing their promoters to a reporter gene and by overexpression of the HtpG protein with three of them. All recombinant vectors exhibited full structural stability.Entities:
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Year: 2005 PMID： 16005967 DOI： 10.1016/j.plasmid.2005.05.001
Source DB: PubMed Journal: Plasmid ISSN： 0147-619X Impact factor: 3.466