Literature DB >> 22100269

Development of a strong intracellular expression system for Bacillus subtilis by optimizing promoter elements.

Trang Thi Phuong Phan1, Hoang Duc Nguyen, Wolfgang Schumann.   

Abstract

Transcription efficiency of inducible promoters remains a bottleneck in recombinant protein production in Bacillus subtilis cells. Here, we present experimental data how to generate strong IPTG-inducible promoters by optimization of nucleotides at the conserved regions of the groESL promoter including the UP element, the -35, -15, -10 and the +1 region. Combination of these changes into one promoter enhanced the amount of recombinant proteins accumulating intracellularly up to about 30% of the total cellular protein.
Copyright © 2011 Elsevier B.V. All rights reserved.

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Year:  2011        PMID： 22100269     DOI： 10.1016/j.jbiotec.2011.10.006

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  26 in total

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Journal:  World J Microbiol Biotechnol       Date:  2018-09-10       Impact factor: 3.312

6.  Construction and development of an auto-regulatory gene expression system in Bacillus subtilis.

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7.  Combinatorial Sec pathway analysis for improved heterologous protein secretion in Bacillus subtilis: identification of bottlenecks by systematic gene overexpression.

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Journal:  Microb Cell Fact       Date:  2015-06-26       Impact factor: 5.328

8.  Development of Pgrac100-based expression vectors allowing high protein production levels in Bacillus subtilis and relatively low basal expression in Escherichia coli.

Authors:  Trang Thi Phuong Phan; Linh Thuoc Tran; Wolfgang Schumann; Hoang Duc Nguyen
Journal:  Microb Cell Fact       Date:  2015-05-21       Impact factor: 5.328

9.  The pCri System: a vector collection for recombinant protein expression and purification.

Authors:  Theodoros Goulas; Anna Cuppari; Raquel Garcia-Castellanos; Scott Snipas; Rudi Glockshuber; Joan L Arolas; F Xavier Gomis-Rüth
Journal:  PLoS One       Date:  2014-11-11       Impact factor: 3.240

10.  Generation of an artificial double promoter for protein expression in Bacillus subtilis through a promoter trap system.

Authors:  Mingming Yang; Weiwei Zhang; Shengyue Ji; Pinghua Cao; Yulin Chen; Xin Zhao
Journal:  PLoS One       Date:  2013-02-08       Impact factor: 3.240
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